ArticleProceedings of the National Academy of Sciences of the United States of America2007
Detection of fractional steps in cargo movement by the collective operation of kinesin-1 motors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 144 citations in OpenAlex.
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- Wave-like oscillations of clamped microtubules driven by collective dynein transport.Biophysical journal · 2024Article
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- Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins.Journal of visualized experiments : JoVE · 2022Article
- Article
- The Tail of Kinesin-14a in Giardia Is a Dual Regulator of Motility.Current biology : CB · 2020Article
- Modelling cytoskeletal transport by clusters of non-processive molecular motors with limited binding sites.Royal Society open science · 2020Article
- High-Precision Protein-Tracking With Interferometric Scattering Microscopy.Frontiers in cell and developmental biology · 2020Article
- Article
- Large and reversible myosin-dependent forces in rigidity sensing.Nature physics · 2019Article
- Force Generated by Two Kinesin Motors Depends on the Load Direction and Intermolecular Coupling.Physical review letters · 2019Article
- Linear motor driven-rotary motion of a membrane-permeabilized ghost in Mycoplasma mobile.Scientific reports · 2018Article
- Motor Reattachment Kinetics Play a Dominant Role in Multimotor-Driven Cargo Transport.Biophysical journal · 2018Article
- Challenges in Estimating the Motility Parameters of Single Processive Motor Proteins.Biophysical journal · 2017Article
- Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement.Biophysical journal · 2017Article
- Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Cargo Transport by Two Coupled Myosin Va Motors on Actin Filaments and Bundles.Biophysical journal · 2016Article
- Label-free Imaging of Microtubules with Sub-nm Precision Using Interferometric Scattering Microscopy.Biophysical journal · 2016Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The stepping behavior of single kinesin-1 motor proteins has been studied in great detail. However, in cells, these motors often do not work alone but rather function in small groups when they transport cellular cargo. Until now, the cooperative interactions between motors in such groups were poorly understood. A fundamental question is whether two or more motors that move the same cargo step in synchrony, producing the same step size as a single motor, or whether the step size of the cargo movement varies. To answer this question, we performed in vitro gliding motility assays, where microtubules coated with quantum dots were driven over a glass surface by a known number of kinesin-1 motors. The motion of individual microtubules was then tracked with nanometer precision. In the case of transport by two kinesin-1 motors, we found successive 4-nm steps, corresponding to half the step size of a single motor. Dwell-time analysis did not reveal any coordination, in the sense of alternate stepping, between the motors. When three motors interacted in collective transport, we identified distinct forward and backward jumps on the order of 10 nm. The existence of the fractional steps as well as the distinct jumps illustrate a lack of synchronization and has implications for the analysis of motor-driven organelle movement investigated in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.