ArticlePlant cell reports2007
Expression of a feedback insensitive anthranilate synthase gene from tobacco increases free tryptophan in soybean plants.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 40 citations in OpenAlex.
- 3-dehydroquinate synthase contributes to anthocyanin accumulation and flower pigmentation in petunia.Protoplasma · 2026Article
- Anthranilate at the interface of tryptophan and specialized metabolite biosynthesis.Frontiers in plant science · 2025Review
- Molecular mapping and genomics of soybean seed protein: a review and perspective for the future.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Review
- Metabolomics of genetically modified crops.International journal of molecular sciences · 2014Review
- Recent advances in soybean transformation and their application to molecular breeding and genomic analysis.Breeding science · 2012Article
- Manipulation of amino acid composition in soybean seeds by the combination of deregulated tryptophan biosynthesis and storage protein deficiency.Plant cell reports · 2010Article
- Tobacco plastid transformation using the feedback-insensitive anthranilate synthase [alpha]-subunit of tobacco (ASA2) as a new selectable marker.Journal of experimental botany · 2009Article
- Tissue culture specificity of the tobacco ASA2 promoter driving hpt as a selectable marker for soybean transformation selection.Plant cell reports · 2008Article
- Improving the content of essential amino acids in crop plants: goals and opportunities.Plant physiology · 2008Review
- Use of 4-methylindole or 7-methyl-DL-tryptophan in a transformant selection system based on the feedback-insensitive anthranilate synthase alpha-subunit of tobacco (ASA2).Plant cell reports · 2008Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soybean [Glycine max (L.) Merr.] embryogenic cultures were transformed by particle bombardment with the feedback-insensitive tobacco anthranilate synthase (AS) gene ASA2 driven by the CaMV 35S promoter and selected using hph as the selectable marker gene. Only one of eight regenerated lines that set seed and contained ASA2 expressed the gene highly and contained increased free tryptophan (Trp) levels in leaves, seeds and embryogenic cultures. Leaf extracts of the ASA2 expressing line contained about twice as much AS enzyme activity as the untransformed control and this activity was only slightly more feedback-insensitive. Amino acid analysis showed that both leaves and embryogenic tissue cultures of the ASA2 expressing line had four to five-times the normal levels of free Trp and slightly higher free tyrosine and phenylalanine. The seed total Trp content was only slightly increased. Metabolic profiling-analysis by GC-MS detected no other consistent differences. These studies show that the ASA2 gene can be expressed in soybean and that modest changes in Trp synthesis occurs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.