ArticleThe Journal of membrane biology2007
The functional interrelationship between gap junctions and fenestrae in endothelial cells of the liver organoid.
Article in The Journal of membrane biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 14 citations in OpenAlex.
- SK-Hep1: not hepatocellular carcinoma cells but a cell model for liver sinusoidal endothelial cells.International journal of clinical and experimental pathology · 2018Article
- Microfluidic-Based Multi-Organ Platforms for Drug Discovery.Micromachines · 2016Review
- In vivo delivery, pharmacokinetics, biodistribution and toxicity of iron oxide nanoparticles.Chemical Society reviews · 2015Review
- Glomerular endothelial cell fenestrations: an integral component of the glomerular filtration barrier.American journal of physiology. Renal physiology · 2009Review
- New insights into the dynamics of sinusoidal endothelial fenestrae in liver sinusoidal endothelial cells.Medical molecular morphology · 2008Review
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional intact liver organoid can be reconstructed in a radial-flow bioreactor when human hepatocellular carcinoma (FLC-5), mouse immortalized sinusoidal endothelial M1 (SEC) and A7 (HSC) hepatic stellate cell lines are cocultured. The structural and functional characteristics of the reconstructed organoid closely resemble the in vivo liver situation. Previous liver organoid studies indicated that cell-to-cell communications might be an important factor for the functional and structural integrity of the reconstructed organoid, including the expression of fenestrae. Therefore, we examined the possible relationship between functional intact gap junctional intercellular communication (GJIC) and fenestrae dynamics in M1-SEC cells. The fine morphology of liver organoid was studied in the presence of (1) irsogladine maleate (IM), (2) oleamide and (3) oleamide followed by IM treatment. Fine ultrastructural changes were studied by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) and compared with control liver organoid data. TEM revealed that oleamide affected the integrity of cell-to-cell contacts predominantly in FLC-5 hepatocytes. SEM observation showed the presence of fenestrae on M1-SEC cells; however, oleamide inhibited fenestrae expression on the surface of endothelial cells. Interestingly, fenestrae reappeared when IM was added after initial oleamide exposure. GJIC mediates the number of fenestrae in endothelial cells of the liver organoid.
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