ArticleMolecular biology of the cell2007
Centrin1 is required for organelle segregation and cytokinesis in Trypanosoma brucei.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 102 citations in OpenAlex.
- Polo-like kinase phosphorylation of the orphan kinesin KIN-G negatively regulates centrin arm biogenesis ineLife · 2026Article
- Characterization of the dual functions of Leishmania CK1.2 in both the parasite and the macrophage.PloS one · 2026Article
- Mechanistic insights into LdCen1-LdDRP interaction facilitating UV-induced DNA damage repair in Leishmania donovani.Medical microbiology and immunology · 2025Article
- An Update on Clinical and Pathogenic Spectra of Leishmaniasis.Expert reviews in molecular medicine · 2025Review
- An orphan kinesin inmBio · 2024Article
- Multiplex detection and identification of viral, bacterial, and protozoan pathogens in human blood and plasma using an expanded high-density resequencing microarray platform.Frontiers in molecular biosciences · 2024Article
- Characterisation of TbSmee1 suggests endocytosis allows surface-bound cargo to enter the trypanosome flagellar pocket.Journal of cell science · 2023Article
- Centrin-deficient Leishmania mexicana confers protection against Old World visceral leishmaniasis.NPJ vaccines · 2022Article
- Preclinical validation of a live attenuated dermotropic Leishmania vaccine against vector transmitted fatal visceral leishmaniasis.Communications biology · 2021Article
- Expression analysis of centrin gene in promastigote and amastigote forms of leishmania infantum iranian isolates: a promising target for live attenuated vaccine development against canine leishmaniasis.BMC veterinary research · 2021Article
- The kinetoplastid-specific phosphatase KPP1 attenuates PLK activity to facilitate flagellum inheritance inScience signaling · 2021Article
- Targeted Deletion of Centrin inFrontiers in cellular and infection microbiology · 2021Article
- Maintenance of hook complex integrity and centrin arm assembly facilitates flagellum inheritance inThe Journal of biological chemistry · 2020Article
- A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing.Nature communications · 2020Article
- Regulated protein stabilization underpins the functional interplay among basal body components inThe Journal of biological chemistry · 2020Article
- Disruption of Intracellular Calcium Homeostasis as a Therapeutic Target AgainstFrontiers in cellular and infection microbiology · 2020Review
- Trypanosoma brucei centrin5 is enriched in the flagellum and interacts with other centrins in a calcium-dependent manner.FEBS open bio · 2019Article
- BOH1 cooperates with Polo-like kinase to regulate flagellum inheritance and cytokinesis initiation inJournal of cell science · 2019Article
- Review
- Flagellum inheritance inThe Journal of biological chemistry · 2018Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centrin is a calcium-binding centrosome/basal body-associated protein involved in duplication and segregation of these organelles in eukaryotes. We had shown that disruption of one of the centrin genes (centrin1) in Leishmania amastigotes resulted in failure of both basal body duplication and cytokinesis. Here, we undertook to define the role of centrin1 (TbCen1) in the duplication and segregation of basal body and its associated organelles kinetoplast and Golgi, as well as its role in cytokinesis of the procyclic form of Trypanosoma brucei by depleting its protein using RNA inhibition methodology. TbCen1-depleted cells showed significant reduction in growth compared with control cells. Morphological analysis of these cells showed they were large and pleomorphic with multiple detached flagella. Both immunofluorescence assays using organelle-specific antibodies and electron microscopic analysis showed that TbCen1-deficient cells contained multiple basal bodies, kinetoplasts, Golgi, and nuclei. These multiple organelles were, however, closely clustered together, indicating duplication without segregation in the absence of centrin. This failure in organelle segregation may be the likely cause of inhibition of cytokinesis, suggesting for the first time a new and unique role for centrin in the segregation of organelles without affecting their multiplication in the procyclic form of T. brucei.
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