ArticleJournal of clinical microbiology2007
Development of real-time PCR assays for genotyping of Chlamydia trachomatis.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- High Burden ofMicroorganisms · 2026Article
- Prevalence and circulant genotypes of Chlamydia trachomatis in university women from cities in the Brazilian Amazon.PloS one · 2024Article
- Identification and characterization of mixed infections ofFrontiers in microbiology · 2022Article
- Article
- Molecular epidemiology and socio-demographic risk factors of sexually transmitted infections among women in Lebanon.BMC infectious diseases · 2020Article
- The Prevalence of HSV, HHV-6, HPV andPathogens (Basel, Switzerland) · 2019Article
- The Finnish New Variant ofMicroorganisms · 2019Article
- Transcriptional Expression of theMicroorganisms · 2019Article
- Chlamydiae in human intestinal biopsy samples.Pathogens and disease · 2018Article
- High prevalence of sexual Chlamydia trachomatis infection in young women from Marajó Island, in the Brazilian Amazon.PloS one · 2018Article
- High Frequency of Chlamydia trachomatis Mixed Infections Detected by Microarray Assay in South American Samples.PloS one · 2016Article
- Genotyping Chlamydia trachomatis strains among men who have sex with men from a Northern Spain region: a cohort study.BMJ open · 2013Article
- Rapid determination of lymphogranuloma venereum serovars of Chlamydia trachomatis by quantitative high-resolution melt analysis (HRMA).Journal of clinical microbiology · 2012Article
- Evaluation of a high resolution genotyping method for Chlamydia trachomatis using routine clinical samples.PloS one · 2011Article
- Chlamydia trachomatis genotypes and the Swedish new variant among urogenital Chlamydia trachomatis strains in Finland.Infectious diseases in obstetrics and gynecology · 2011Article
- Development and evaluation of an ompA quantitative real-time PCR assay for Chlamydia trachomatis serovar determination.Journal of clinical microbiology · 2010Article
- Rapid detection of Chlamydia trachomatis and typing of the Lymphogranuloma venereum associated L-Serovars by TaqMan PCR.BMC infectious diseases · 2008Article
- Genotyping of Chlamydia trachomatis by microsphere suspension array.Journal of clinical microbiology · 2008Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and LGV reference strains and did not cross-react with the DNA from 26 strains representing the bacterial pathogens and commensals of the oropharynx, genital tract, and rectum. The NRT-PCR had a 95% probability of detection at four genome copies (confidence interval, three to six copies) of C. trachomatis per reaction. One hundred cervical and urethral swab specimens containing C. trachomatis DNA from 63 women and 37 men were used to validate the method. The results from the NRT-PCR and the DNA sequencing of amplicons generated from the omp1 gene showed 100% correlation for these samples. The assay also identified the LGV-II genotype in 24 of 48 rectal swab specimens containing C. trachomatis DNA that were obtained from men having sex with men. The Sexually Transmitted Bacteria Reference Laboratory, London, independently confirmed these results using group-specific LGV real-time PCR and restriction fragment length polymorphism analysis. Compared with the NRT-PCR, non-NRT-PCR was found to be less sensitive: it typed C. trachomatis DNA in only 80% of the genital samples and 90% of the rectal swab samples. This is the first successful demonstration of the use of real-time PCR for the genotype-specific typing of C. trachomatis strains that cause sexually transmitted diseases.
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