ArticleThe EMBO journal1991
DNA replication in cell-free extracts from Drosophila melanogaster.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Engineered SUMO/protease system identifies Pdr6 as a bidirectional nuclear transport receptor.The Journal of cell biology · 2019Article
- Chromatin remodeling in Drosophila preblastodermic embryo extract.Scientific reports · 2018Article
- Dynamic loading and redistribution of the Mcm2-7 helicase complex through the cell cycle.The EMBO journal · 2015Article
- Fates-shifted is an F-box protein that targets Bicoid for degradation and regulates developmental fate determination in Drosophila embryos.Nature cell biology · 2011Article
- Study of DNA replication in Drosophila using cell free in vitro system.Cell cycle (Georgetown, Tex.) · 2010Article
- Role of the Orc6 protein in origin recognition complex-dependent DNA binding and replication in Drosophila melanogaster.Molecular and cellular biology · 2007Article
- Functional analysis of mutant and wild-type Drosophila origin recognition complex.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Drosophila ORC specifically binds to ACE3, an origin of DNA replication control element.Genes & development · 1999Article
- Assembly of functionally active Drosophila origin recognition complex from recombinant proteins.Genes & development · 1999Article
- Nuclear membrane vesicle targeting to chromatin in a Drosophila embryo cell-free system.Molecular biology of the cell · 1997Article
- Cell cycle regulation of the replication licensing system: involvement of a Cdk-dependent inhibitor.The Journal of cell biology · 1997Article
- ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae.The EMBO journal · 1996Article
- Fission yeast cdc21, a member of the MCM protein family, is required for onset of S phase and is located in the nucleus throughout the cell cycle.The EMBO journal · 1996Article
- Purification and characterisation of a DNA helicase, dheI I, from Drosophila melanogaster embryos.Nucleic acids research · 1995Article
- dpa, a member of the MCM family, is required for mitotic DNA replication but not endoreplication in Drosophila.The EMBO journal · 1995Article
- Article
- DF 31, a sperm decondensation factor from Drosophila melanogaster: purification and characterization.The EMBO journal · 1995Article
- Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor.The Journal of cell biology · 1993Article
- Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed an efficient in vitro replication system from 0-2 h Drosophila melanogaster embryos. Demembranated Xenopus sperm DNA when incubated in such an extract first becomes enclosed in a nucleus-like structure with a nuclear envelope and a karyoskeleton. It then undergoes one round of semiconservative replication--this replication appears completely dependent on nuclear formation. Up to 30% of input DNA is nucleated in one reaction. Efficient nuclear formation and replication are dependent on a cold treatment step, prior to disruption of the embryos. They also depend on the age of the embryos used. Extracts from older embryos (0-5 h) are capable of nuclear formation, although at a much reduced efficiency, and repair synthesis, but seem to have lost the ability to initiate DNA replication. In addition to replicating sperm DNA this system appears capable of carrying out semi-conservative replication on some plasmids. However, it cannot use these to trigger nuclear formation; replication is only seen if the plasmids are coincubated with sperm DNA. The in vitro formed nuclei have not been observed to trigger nuclear envelope breakdown and entry into mitosis. However, they can re-replicate the DNA if the nuclei are permeabilized. This system should be a useful complement to the previously isolated Xenopus in vitro replication system. In addition the amenability of Drosophila to genetic study should open up new approaches not previously possible with Xenopus.
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