ArticlePloS one2007
Genome-wide identification of alternatively spliced mRNA targets of specific RNA-binding proteins.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Noncanonical function of the Sex lethal gene controls the protogyny phenotype in Drosophila melanogaster.Scientific reports · 2022Article
- The Regulation of Germline Sex Determination in Drosophila by Sex lethal.Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation · 2022Review
- Interplay between sex determination cascade and major signaling pathways during Drosophila eye development: Perspectives for future research.Developmental biology · 2021Review
- Genomic and cDNA selection-amplification identifies transcriptome-wide binding sites for the Drosophila protein sex-lethal.PloS one · 2021Article
- A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates.Journal of visualized experiments : JoVE · 2018Article
- Article
- The sex of specific neurons controls female body growth in Drosophila.PLoS biology · 2017Article
- Promiscuity in post-transcriptional control of gene expression: Drosophila sex-lethal and its regulatory partnerships.FEBS letters · 2017Review
- Article
- Sex-lethal enables germline stem cell differentiation by down-regulating Nanos protein levels during Drosophila oogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Molecular evolution of Drosophila Sex-lethal and related sex determining genes.BMC evolutionary biology · 2012Article
- Distinct regulatory programs establish widespread sex-specific alternative splicing in Drosophila melanogaster.RNA (New York, N.Y.) · 2011Article
- Integration of a splicing regulatory network within the meiotic gene expression program of Saccharomyces cerevisiae.Genes & development · 2010Article
- Sex-specific splicing in Drosophila: widespread occurrence, tissue specificity and evolutionary conservation.Genetics · 2009Article
- Global analysis of mRNA splicing.RNA (New York, N.Y.) · 2008Review
- Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlternative splicing plays an important role in generating molecular and functional diversity in multi-cellular organisms. RNA binding proteins play crucial roles in modulating splice site choice. The majority of known binding sites for regulatory proteins are short, degenerate consensus sequences that occur frequently throughout the genome. This poses an important challenge to distinguish between functionally relevant sequences and a vast array of those occurring by chance. METHODOLOGY/PRINCIPAL
findingsHere we have used a computational approach that combines a series of biological constraints to identify uridine-rich sequence motifs that are present within relevant biological contexts and thus are potential targets of the Drosophila master sex-switch protein Sex-lethal (SXL). This strategy led to the identification of one novel target. Moreover, our systematic analysis provides a starting point for the molecular and functional characterization of an additional target, which is dependent on SXL activity, either directly or indirectly, for regulation in a germline-specific manner. CONCLUSIONS/SIGNIFICANCE: This approach has successfully identified previously known, new, and potential SXL targets. Our analysis suggests that only a subset of potential SXL sites are regulated by SXL. Finally, this approach should be directly relevant to the large majority of splicing regulatory proteins for which bonafide targets are unknown.
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