ArticleMolecular genetics and genomics : MGG2007
Interaction between the ribosomal subunits: 16S rRNA suppressors of the lethal DeltaA1916 mutation in the 23S rRNA of Escherichia coli.
Article in Molecular genetics and genomics : MGG, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Pseudomonas aeruginosa cleaves the decoding center of Caenorhabditis elegans ribosomes.PLoS biology · 2020Article
- Inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function in wild-type Escherichia coli and Salmonella enterica.Journal of bacteriology · 2011Article
- Helix 69 in 23S rRNA modulates decoding by wild type and suppressor tRNAs.Molecular genetics and genomics : MGG · 2009Article
- Mutations in conserved helix 69 of 23S rRNA of Thermus thermophilus that affect capreomycin resistance but not posttranscriptional modifications.Journal of bacteriology · 2008Article
- YbeA is the m3Psi methyltransferase RlmH that targets nucleotide 1915 in 23S rRNA.RNA (New York, N.Y.) · 2008Article
- pH-dependent structural changes of helix 69 from Escherichia coli 23S ribosomal RNA.RNA (New York, N.Y.) · 2008Article
- Expanding the nucleotide repertoire of the ribosome with post-transcriptional modifications.ACS chemical biology · 2007Review
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A1916 in 23S rRNA is located in one of the major intersubunit bridges of the 70S ribosome. Deletion of A1916 disrupts the intersubunit bridge B2a, promotes misreading of the genetic code and is lethal. In a genetic selection for suppressor mutations, two base substitutions in 16S rRNA were recovered that restored viability and also allowed expression of DeltaA1916-associated capreomycin resistance. These mutations were G1048A in helix 34 and U1471C in helix 44. Restoration of function is incomplete, however, and the double mutants are slow-growing, defective in subunit association and support high levels of translational errors. In contrast, none of these parameters is affected by the single 16S suppressor mutations. U1471C likely affects another intersubunit contact, bridge B6, suggesting that interactions between different bridges and cross-talk between subunits contributes to ribosomal function.
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