ArticleProceedings of the National Academy of Sciences of the United States of America2007
Automated comparative sequence analysis by base-specific cleavage and mass spectrometry for nucleic acid-based microbial typing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 83 citations in OpenAlex.
- "Omic" Approaches to Bacteria and Antibiotic Resistance Identification.International journal of molecular sciences · 2022Review
- Unraveling the RNA modification code with mass spectrometry.Molecular omics · 2020Article
- Article
- Mass spectrometry-based comparative sequence analysis for the genetic monitoring of influenza A(H1N1)pdm09 virus.PloS one · 2014Article
- Emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management.BioMed research international · 2014Review
- Application of mass spectrometry to molecular diagnostics of viral infections.Expert review of molecular diagnostics · 2013Review
- Application of maldi-tof mass spectrometry in clinical virology: a review.The open virology journal · 2013Article
- [Mass spectrometry in the clinical microbiology laboratory].Enfermedades infecciosas y microbiologia clinica · 2012Review
- Probing mixed-genotype infections I: extraction and cloning of infections from hosts of the trypanosomatid Crithidia bombi.PloS one · 2012Article
- Multilocus sequence typing of Streptococcus pneumoniae by use of mass spectrometry.Journal of clinical microbiology · 2011Article
- Bacterial epidemiology and biology--lessons from genome sequencing.Genome biology · 2011Review
- Call for a quality standard for sequence-based assays in clinical microbiology: necessity for quality assessment of sequences used in microbial identification and typing.Journal of clinical microbiology · 2011Review
- Robust hepatitis B virus genotyping by mass spectrometry.Journal of clinical microbiology · 2010Article
- Replacing reverse line blot hybridization spoligotyping of the Mycobacterium tuberculosis complex.Journal of clinical microbiology · 2010Article
- Molecular complexity of successive bacterial epidemics deconvoluted by comparative pathogenomics.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Mass spectrometry tools for the classification and identification of bacteria.Nature reviews. Microbiology · 2010Review
- [Microbiological diagnosis: Etiologic driven and syndrome driven].Antibiotiques (Paris, France : 1999) · 2009Article
- Direct bacterial profiling by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for identification of pathogenic Neisseria.The Journal of molecular diagnostics : JMD · 2009Article
- Classification and identification of bacteria by mass spectrometry and computational analysis.PloS one · 2008Article
- High-throughput genotyping of Salmonella enterica serovar Typhi allowing geographical assignment of haplotypes and pathotypes within an urban District of Jakarta, Indonesia.Journal of clinical microbiology · 2008Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Traditional microbial typing technologies for the characterization of pathogenic microorganisms and monitoring of their global spread are often difficult to standardize and poorly portable, and they lack sufficient ease of use, throughput, and automation. To overcome these problems, we introduce the use of comparative sequencing by MALDI-TOF MS for automated high-throughput microbial DNA sequence analysis. Data derived from the public multilocus sequence typing (MLST) database (http://pubmlst.org/neisseria) established a reference set of expected peak patterns. A model pathogen, Neisseria meningitidis, was used to validate the technology and explore its applicability as an alternative to dideoxy sequencing. One hundred N. meningitidis samples were typed by comparing MALDI-TOF MS fingerprints of the standard MLST loci to reference sequences available in the public MLST database. Identification results can be obtained in 2 working days. Results were in concordance with classical dideoxy sequencing with 98% correct automatic identification. Sequence types (STs) of 89 samples were represented in the database, seven samples revealed new STs, including three new alleles, and four samples contained mixed populations of multiple STs. The approach shows interlaboratory reproducibility and allows for the exchange of mass spectrometric fingerprints to study the geographic spread of epidemic N. meningitidis strains or other microbes of clinical importance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.