ArticleMolecular and cellular biology2007
Sequence dependence of chromosomal R-loops at the immunoglobulin heavy-chain Smu class switch region.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
- Nucleotide level mapping of uracils in murine heavy chain switch regions shows correlation between uracilation and positions of switch junctions created during class-switch recombination.NAR molecular medicine · 2025Article
- Cooperativity between Cas9 and hyperactive AID establishes broad and diversifying mutational footprints in base editors.Nucleic acids research · 2024Article
- Senataxin and RNase H2 act redundantly to suppress genome instability during class switch recombination.eLife · 2022Article
- Review
- Mapping R-Loops and RNA:DNA Hybrids with S9.6-Based Immunoprecipitation Methods.Journal of visualized experiments : JoVE · 2021Article
- Emerging roles for R-loop structures in the management of topological stress.The Journal of biological chemistry · 2020Review
- Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation.Journal of molecular biology · 2020Article
- Current insights into the mechanism of mammalian immunoglobulin class switch recombination.Critical reviews in biochemistry and molecular biology · 2019Review
- Early detection of pre-malignant lesions in a KRASDisease models & mechanisms · 2019Article
- Novel Lines of Evidence for the Asymmetric Strand Displacement Model of Mitochondrial DNA Replication.Molecular and cellular biology · 2019Article
- RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.Molecular cell · 2018Article
- Canonical DNA Repair Pathways Influence R-Loop-Driven Genome Instability.Journal of molecular biology · 2017Article
- DNA-RNA hybrids: the risks of DNA breakage during transcription.Nature structural & molecular biology · 2017Article
- A transcriptional serenAID: the role of noncoding RNAs in class switch recombination.International immunology · 2017Review
- Physiological Roles of DNA Double-Strand Breaks.Journal of nucleic acids · 2017Review
- Nascent Connections: R-Loops and Chromatin Patterning.Trends in genetics : TIG · 2016Review
- Transcription-coupled genetic instability marks acute lymphoblastic leukemia structural variation hotspots.eLife · 2016Article
- AID hits the jackpot when missing the target.Current opinion in immunology · 2016Review
- Activation-induced deoxycytidine deaminase (AID) co-transcriptional scanning at single-molecule resolution.Nature communications · 2015Article
- The repetitive portion of the Xenopus IgH Mu switch region mediates orientation-dependent class switch recombination.Molecular immunology · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The mechanism by which the cytidine deaminase activation-induced deaminase (AID) acts at immunoglobulin heavy-chain class switch regions during mammalian class switch recombination (CSR) remains unclear. R-loops have been proposed as a basis for this targeting. Here, we show that the difference between various forms of the Smu locus that can or cannot undergo CSR correlates well with the locations and detectability of R-loops. The Smu R-loops can initiate hundreds of base pairs upstream of the core repeat switch regions, and the area where the R-loops initiate corresponds to the zone where the AID mutation frequency begins to rise, despite a constant density of WRC sites in this region. The frequency of R-loops is 1 in 25 alleles, regardless of the presence of the core Smu repeats, again consistent with the initiation of most R-loops upstream of the core repeats. These findings explain the surprisingly high levels of residual CSR in B cells from mice lacking the core Smu repeats but the marked reduction in CSR in mice with deletions of the region upstream of the core Smu repeats. These studies also provide the first analysis of how R-loop formation in the eukaryotic chromosome depends on the DNA sequence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.