ArticleInfection and immunity2007
Identification of gene products involved in biofilm production by Moraxella catarrhalis ETSU-9 in vitro.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- A global perspective on the genomics ofMicrobial genomics · 2025Article
- Correlation ofEmerging microbes & infections · 2022Article
- Utilization of Variant and Fusion Proteins To Functionally Map the Aggregatibacter actinomycetemcomitans Trimeric Autotransporter Protein ApiA.Infection and immunity · 2018Article
- A Moraxella catarrhalis two-component signal transduction system necessary for growth in liquid media affects production of two lysozyme inhibitors.Infection and immunity · 2015Article
- The Autotransporter BpaB Contributes to the Virulence of Burkholderia mallei in an Aerosol Model of Infection.PloS one · 2015Article
- Identification of an outer membrane lipoprotein involved in nasopharyngeal colonization by Moraxella catarrhalis in an animal model.Infection and immunity · 2014Article
- Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei.BMC microbiology · 2014Article
- Moraxella catarrhalis uses a twin-arginine translocation system to secrete the β-lactamase BRO-2.BMC microbiology · 2013Article
- Panel 5: Microbiology and immunology panel.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2013Review
- Future perspective on host-pathogen interactions during bacterial biofilm formation within the nasopharynx.Future microbiology · 2012Review
- Identification of the genes involved in Riemerella anatipestifer biofilm formation by random transposon mutagenesis.PloS one · 2012Article
- Identification of gene products involved in the oxidative stress response of Moraxella catarrhalis.Infection and immunity · 2011Article
- Identification of Burkholderia mallei and Burkholderia pseudomallei adhesins for human respiratory epithelial cells.BMC microbiology · 2010Article
- Hag mediates adherence of Moraxella catarrhalis to ciliated human airway cells.Infection and immunity · 2009Article
- Identification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis.BMC microbiology · 2009Article
- Molecular aspects of Moraxella catarrhalis pathogenesis.Microbiology and molecular biology reviews : MMBR · 2009Review
- Modular arrangement of allelic variants explains the divergence in Moraxella catarrhalis UspA protein function.Infection and immunity · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Moraxella catarrhalis ETSU-9 was subjected to random transposon insertion mutagenesis to identify genes encoding products involved in the ability of the organism to form biofilms in vitro. Screening of approximately 3,000 transposon insertion mutants in the crystal violet-based biofilm assay system yielded six mutants that exhibited greatly reduced abilities to form biofilms. Three of these mutants had transposon insertions in the uspA2H gene, which encodes a surface protein previously shown to be involved in the ability of M. catarrhalis to both attach to human cell lines in vitro and resist killing by normal human serum. Random insertion mutagenesis of the uspA2H gene, involving the introduction of a 15-nucleotide fragment encoding 5 amino acids, was used to attempt to identify the domain(s) necessary for biofilm formation. Most of these insertions adversely affected biofilm formation, whereas the abilities of these same mutants to attach to Chang conjunctival epithelial cells in vitro were usually not reduced. Gain-of-function experiments showed that introduction of the M. catarrhalis ETSU-9 uspA2H gene into Escherichia coli conferred biofilm formation ability on this recombinant strain. Two of the other three M. catarrhalis ETSU-9 transposon insertion mutants that had greatly reduced abilities to form biofilms were shown to have insertions in genes encoding products predicted to be directly or indirectly involved in cell wall metabolism.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.