ArticleThe Journal of biological chemistry2007
Active ADP-ribosylation factor-1 (ARF1) is required for mitotic Golgi fragmentation.
Article in The Journal of biological chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Reevaluating Golgi fragmentation and its implications in wound repair.Cell regeneration (London, England) · 2024Review
- Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- HPV is a cargo for the COPI sorting complex during virus entry.Science advances · 2023Article
- Common Assays in Mammalian Golgi Studies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish.Nature communications · 2022Article
- Neuronal accumulation of peroxidated lipids promotes demyelination and neurodegeneration through the activation of the microglial NLRP3 inflammasome.Nature aging · 2021Article
- Review
- GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion.Autophagy · 2019Article
- Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase M.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Golgi structure formation, function, and post-translational modifications in mammalian cells.F1000Research · 2017Review
- Cell cycle regulation of Golgi membrane dynamics.Trends in cell biology · 2013Review
- Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65.Nature communications · 2013Article
- Sequential phosphorylation of GRASP65 during mitotic Golgi disassembly.Biology open · 2012Article
- Golgin160 recruits the dynein motor to position the Golgi apparatus.Developmental cell · 2012Article
- Quantitative analysis of liver Golgi proteome in the cell cycle.Methods in molecular biology (Clifton, N.J.) · 2012Article
- PLCζ causes Ca(2+) oscillations in mouse eggs by targeting intracellular and not plasma membrane PI(4,5)P(2).Molecular biology of the cell · 2012Article
- The ubiquitin ligase HACE1 regulates Golgi membrane dynamics during the cell cycle.Nature communications · 2011Article
- Golgi biogenesis.Cold Spring Harbor perspectives in biology · 2011Review
- The role of GRASP65 in Golgi cisternal stacking and cell cycle progression.Traffic (Copenhagen, Denmark) · 2010Article
- Role of the GTPase Rab1b in ebolavirus particle formation.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In mammalian cells the Golgi apparatus undergoes an extensive disassembly process at the onset of mitosis that is believed to facilitate equal partitioning of this organelle into the two daughter cells. However, the underlying mechanisms for this fragmentation process are so far unclear. Here we have investigated the role of the ADP-ribosylation factor-1 (ARF1) in this process to determine whether Golgi fragmentation in mitosis is mediated by vesicle budding. ARF1 is a small GTPase that is required for COPI vesicle formation from the Golgi membranes. Treatment of Golgi membranes with mitotic cytosol or with purified coatomer together with wild type ARF1 or its constitutive active form, but not the inactive mutant, converted the Golgi membranes into COPI vesicles. ARF1-depleted mitotic cytosol failed to fragment Golgi membranes. ARF1 is associated with Golgi vesicles generated in vitro and with vesicles in mitotic cells. In addition, microinjection of constitutive active ARF1 did not affect mitotic Golgi fragmentation or cell progression through mitosis. Our results show that ARF1 is active during mitosis and that this activity is required for mitotic Golgi fragmentation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.