ArticleBritish journal of pharmacology2007
Stimulation of rat erythrocyte P2X7 receptor induces the release of epoxyeicosatrienoic acids.
Article in British journal of pharmacology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 73 citations in OpenAlex.
- Hemodialysis and erythrocyte epoxy fatty acids.Physiological reports · 2020Trial
- Effect of cardiopulmonary bypass on plasma and erythrocytes oxylipins.Lipids in health and disease · 2023Observational
- Maximal exercise and erythrocyte epoxy fatty acids: a lipidomics study.Physiological reports · 2019Article
- Role of pannexin-1 in the cellular uptake, release and hydrolysis of anandamide by T84 colon cancer cells.Scientific reports · 2019Article
- P2X7 ionotropic receptor is functionally expressed in rabbit articular chondrocytes and mediates extracellular ATP cytotoxicity.Purinergic signalling · 2018Article
- Ventilatory and cerebrovascular regulation and integration at high-altitude.Clinical autonomic research : official journal of the Clinical Autonomic Research Society · 2018Review
- Cytochrome P450 eicosanoids in cerebrovascular function and disease.Pharmacology & therapeutics · 2017Review
- Article
- Hypoxemia, oxygen content, and the regulation of cerebral blood flow.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Review
- Blood cells: an historical account of the roles of purinergic signalling.Purinergic signalling · 2015Review
- Connexin and pannexin signaling pathways, an architectural blueprint for CNS physiology and pathology?Cellular and molecular life sciences : CMLS · 2015Review
- ATP release through pannexon channels.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Pannexin 1 channels mediate the release of ATP into the lumen of the rat urinary bladder.The Journal of physiology · 2015Article
- P2X and P2Y receptor signaling in red blood cells.Frontiers in molecular biosciences · 2015Review
- Intrinsic properties and regulation of Pannexin 1 channel.Channels (Austin, Tex.) · 2014Review
- The pannexins: past and present.Frontiers in physiology · 2014Review
- Probenecid protects against transient focal cerebral ischemic injury by inhibiting HMGB1 release and attenuating AQP4 expression in mice.Neurochemical research · 2014Article
- The bizarre pharmacology of the ATP release channel pannexin1.Neuropharmacology · 2013Review
- Arachidonic acid closes innexin/pannexin channels and thereby inhibits microglia cell movement to a nerve injury.Developmental neurobiology · 2013Article
- P2X7 receptor activation induces reactive oxygen species formation in erythroid cells.Purinergic signalling · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
background and purposeRed blood cells (RBCs) are reservoirs of vasodilatory, antiaggregatory, and antiinflammatory lipid mediators-epoxyeicosatrienoic acids (EETs). This study addresses the formation and release of erythrocyte-derived EETs in response to ATP receptor stimulation that may represent an important mechanism regarding circulatory regulation. EXPERIMENTAL APPROACH: Erythrocyte EET formation and release were investigated by incubating rat RBCs in physiological salt solution with agents that effected ATP release via P2 receptor stimulation of phospholipase A2 and epoxygenase-like activities with activation of the ATP secretory mechanism. EETs were analyzed by gas and liquid chromatography-mass spectrometry. KEY
resultsEETs were released from rat RBCs: 14,15-, 11,12-, 8,9- and 5,6-EETs in a ratio of 1.2:1.0:0.9:0.8. EETs were produced by epoxidation of arachidonic acid catalyzed by hemoglobin. Spontaneous release of EETs, 0.66+/-0.14 ng per 10(9) RBCs, was dose-dependently increased by an ATP analog, BzATP, and inhibited by P2X(7) receptor antagonists. 5 microM ATP increased release of EETs over 20% to 0.83+/-0.15 ng per 10(9) RBCs; 10 microM BzATP tripled the amount of EET release to 1.87+/-0.20 ng per 10(9) RBCs. EET release by ATP or BzATP was not associated with hemolysis. Carbenoxolone, a gap junction inhibitor that inhibits ATP release, and glibenclamide, an inhibitor of the cystic fibrosis transmembrane conductance regulator (CFTR), which is required for ATP release, inhibited the spontaneous and stimulated EET release from RBCs. CONCLUSIONS AND IMPLICATIONS: EETs are produced and released from RBCs via a mechanism that is mediated by ATP stimulation of P2X(7) receptors coupled to ATP transporters, pannexin-1 and CFTR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.