ArticleApplied and environmental microbiology2007
Real-time reverse transcription-PCR for transcriptional expression analysis of virulence and housekeeping genes in viable but nonculturable Vibrio parahaemolyticus after recovery of culturability.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- Comparative evaluation and validation of rapid quantification methods for Mycoplasma hyopneumoniae: development of a PMA-based viability qPCR assay.Scientific reports · 2026Article
- Viable but non-cultivable state in oral microbiota: a critical review of an underexplored microbial survival strategy.Frontiers in cellular and infection microbiology · 2025Review
- A Photonic Immunosensor Detection Method for Viable and Non-ViableMicroorganisms · 2024Article
- Response mechanism of Vibrio parahaemolyticus at high pressure revealed by transcriptomic analysis.Applied microbiology and biotechnology · 2022Article
- Bacterial dormancy: A subpopulation of viable but non-culturable cells demonstrates better fitness for revival.PLoS pathogens · 2021Article
- Comparative Survival and the Cold-Induced Gene Expression of Pathogenic and NonpathogenicInternational journal of environmental research and public health · 2020Article
- Attenuation of MultipleFrontiers in microbiology · 2019Article
- Establishment and Validation of RNA-Based Predictive Models for Understanding Survival of Vibrio parahaemolyticus in Oysters Stored at Low Temperatures.Applied and environmental microbiology · 2017Article
- Effect of Dietary Minerals on Virulence Attributes ofFrontiers in microbiology · 2017Article
- Metabolome response to temperature-induced virulence gene expression in two genotypes of pathogenic Vibrio parahaemolyticus.BMC microbiology · 2016Article
- Induction of Viable but Nonculturable State in Rhodococcus and Transcriptome Analysis Using RNA-seq.PloS one · 2016Article
- Post-Genomic Analysis of Members of the Family Vibrionaceae.Microbiology spectrum · 2015Review
- Investigation of Reference Genes in Vibrio parahaemolyticus for Gene Expression Analysis Using Quantitative RT-PCR.PloS one · 2015Article
- Insights into Vibrio parahaemolyticus CHN25 response to artificial gastric fluid stress by transcriptomic analysis.International journal of molecular sciences · 2014Article
- Induction, resuscitation and quantitative real-time polymerase chain reaction analyses of viable but nonculturable Vibrio vulnificus in artificial sea water.World journal of microbiology & biotechnology · 2014Article
- Interspecific quorum sensing mediates the resuscitation of viable but nonculturable vibrios.Applied and environmental microbiology · 2014Article
- The importance of the viable but non-culturable state in human bacterial pathogens.Frontiers in microbiology · 2014Review
- Fitness factors in vibrios: a mini-review.Microbial ecology · 2013Review
- Growth and virulence properties of biofilm-forming Salmonella enterica serovar typhimurium under different acidic conditions.Applied and environmental microbiology · 2010Article
- Comparison of the pathogenic potentials of environmental and clinical vibrio parahaemolyticus strains indicates a role for temperature regulation in virulence.Applied and environmental microbiology · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time reverse transcription-PCR method was developed to determine whether the recovery of culturability of viable but nonculturable (VBNC) Vibrio parahaemolyticus induced the expression of virulence genes coding for the thermostable direct hemolysin and for type III secretion system 2 (TTSS2). The culturability of clinical strain Vp5 of V. parahaemolyticus in artificial seawater at 4 degrees C was monitored, and the VBNC state was obtained. One day after entry into the VBNC state, temperature upshifts to 20 and 37 degrees C allowed the recovery of culturability. Standard curves for the relative quantification of expression of the housekeeping genes rpoS, pvsA, fur, and pvuA; the tdh2 gene; and the TTSS2 genes (VPA1354, VPA1346, and VPA1342) were established. The levels of expression of the pvsA and pvuA genes were stable and were used to normalize the levels of expression of the other genes. No transcriptional induction of the selected virulence genes under the temperature conditions used to recover the culturability of the VBNC bacteria was observed. The study results demonstrate that the recovery of culturability of VBNC cells of pathogenic V. parahaemolyticus is restricted to regrowth, without correlation with the induction of virulence gene expression. Disease induction would depend mainly on host-pathogen interactions that allow the expression of the virulence genes. This is the first time that the use of mRNA to detect viable cells was evaluated by computing the half-lives of multiple mRNA species under conditions inducing the VBNC state.
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