ArticleNucleic acids research2007
Relative abundance of the human mitochondrial transcription system and distinct roles for h-mtTFB1 and h-mtTFB2 in mitochondrial biogenesis and gene expression.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 116 citations in OpenAlex.
- Review
- Metabolic environment-driven remodeling of mitochondrial ribosomes regulates translation and biogenesis.Molecular cell · 2025Article
- Assessing the Role of Post-Translational Modifications of Mitochondrial RNA Polymerase.International journal of molecular sciences · 2023Article
- 35 Years of TFAM Research: Old Protein, New Puzzles.Biology · 2023Review
- SUV3 Helicase and Mitochondrial Homeostasis.International journal of molecular sciences · 2023Review
- Mesoscale structure-function relationships in mitochondrial transcriptional condensates.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Phase separation drives the self-assembly of mitochondrial nucleoids for transcriptional modulation.Nature structural & molecular biology · 2021Article
- MicroRNA-574 regulates FAM210A expression and influences pathological cardiac remodeling.EMBO molecular medicine · 2021Article
- Review
- Phosphorylation of mitochondrial transcription factor B2 controls mitochondrial DNA binding and transcription.Biochemical and biophysical research communications · 2020Article
- Article
- Limited predictive value of TFAM in mitochondrial biogenesis.Mitochondrion · 2019Article
- The mitochondrial transcription machinery genes are upregulated in acute myeloid leukemia and associated with poor clinical outcome.Metabolism open · 2019Article
- Transcription, Processing, and Decay of Mitochondrial RNA in Health and Disease.International journal of molecular sciences · 2019Review
- A common pattern of DNase I footprinting throughout the human mtDNA unveils clues for a chromatin-like organization.Genome research · 2018Article
- An unexpected role for the transcriptional coactivator isoform NT-PGC-1α in the regulation of mitochondrial respiration in brown adipocytes.The Journal of biological chemistry · 2017Article
- Human mitochondrial transcription factors TFAM and TFB2M work synergistically in promoter melting during transcription initiation.Nucleic acids research · 2017Article
- Mitochondrial transcription in mammalian cells.Frontiers in bioscience (Landmark edition) · 2017Review
- Multi-focal control of mitochondrial gene expression by oncogenic MYC provides potential therapeutic targets in cancer.Oncotarget · 2016Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Human mitochondrial transcription requires the bacteriophage-related RNA polymerase, POLRMT, the mtDNA-binding protein, h-mtTFA/TFAM, and two transcription factors/rRNA methyltransferases, h-mtTFB1 and h-mtTFB2. Here, we determined the steady-state levels of these core transcription components and examined the consequences of purposeful elevation of h-mtTFB1 or h-mtTFB2 in HeLa cells. On a per molecule basis, we find an approximately 6-fold excess of POLRMT to mtDNA and approximately 3-fold more h-mtTFB2 than h-mtTFB1. We also estimate h-mtTFA at approximately 50 molecules/mtDNA, a ratio predicted to support robust transcription, but not to coat mtDNA. Consistent with a role for h-mtTFB2 in transcription and transcription-primed replication, increased mitochondrial DNA and transcripts result from its over-expression. This is accompanied by increased translation rates of most, but not all mtDNA-encoded proteins. Over-expression of h-mtTFB1 did not significantly influence these parameters, but did result in increased mitochondrial biogenesis. Furthermore, h-mtTFB1 mRNA and protein are elevated in response to h-mtTFB2 over-expression, suggesting the existence of a retrograde signal to the nucleus to coordinately regulate expression of these related factors. Altogether, our results provide a framework for understanding the regulation of human mitochondrial transcription in vivo and define distinct roles for h-mtTFB1 and h-mtTFB2 in mitochondrial biogenesis and gene expression that together likely fine-tune mitochondrial function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.