ArticleThe American journal of tropical medicine and hygiene2007
Use of multiple displacement amplification to increase the detection and genotyping of trypanosoma species samples immobilized on FTA filters.
Article in The American journal of tropical medicine and hygiene, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Whole-genome amplification as a tool to improve bacterial detection by PCR in microbiological samples after endodontic treatment.Frontiers in oral health · 2025Article
- An evaluation of African animal trypanosomiasis control strategies in remote communities of Eastern Zambia.Parasitology · 2024Article
- The detection of African trypanosomes in goats reared in tsetse infested villages of Eastern Zambia.Tropical animal health and production · 2022Article
- Diagnosis of animal trypanosomoses: proper use of current tools and future prospects.Parasites & vectors · 2022Review
- Challenges in the Diagnostic Performance of Parasitological and Molecular Tests in the Surveillance of African Trypanosomiasis in Eastern Zambia.Tropical medicine and infectious disease · 2021Article
- Relationship between Trypanosoma brucei rhodesiense genetic diversity and clinical spectrum among sleeping sickness patients in Uganda.BMC research notes · 2017Article
- Population genetic structure and temporal stability among Trypanosoma brucei rhodesiense isolates in Uganda.Parasites & vectors · 2016Article
- Population genetics of Trypanosoma brucei rhodesiense: clonality and diversity within and between foci.PLoS neglected tropical diseases · 2013Article
- Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- Constraints to estimating the prevalence of trypanosome infections in East African zebu cattle.Parasites & vectors · 2010Article
- Genotypic status of the TbAT1/P2 adenosine transporter of Trypanosoma brucei gambiense isolates from Northwestern Uganda following melarsoprol withdrawal.PLoS neglected tropical diseases · 2009Article
- Efficient genotyping of Schistosoma mansoni miracidia following whole genome amplification.Molecular and biochemical parasitology · 2009Article
- Article
- Trypanosomosis in The Gambia: prevalence in working horses and donkeys detected by whole genome amplification and PCR, and evidence for interactions between trypanosome species.BMC veterinary research · 2008Article
- Spatially and genetically distinct African Trypanosome virulence variants defined by host interferon-gamma response.The Journal of infectious diseases · 2007Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Whole genome amplification methods are a recently developed tool for amplifying DNA from limited template. We report its application in trypanosome infections, characterized by low parasitemias. Multiple displacement amplification (MDA) amplifies DNA with a simple in vitro step and was evaluated on mouse blood samples on FTA filter cards with known numbers of Trypanosoma brucei parasites. The data showed a 20-fold increase in the number of PCRs possible per sample, using primers diagnostic for the multicopy ribosomal ITS region or 177-bp repeats, and a 20-fold increase in sensitivity over nested PCR against a single-copy microsatellite. Using MDA for microsatellite genotyping caused allele dropout at low DNA concentrations, which was overcome by pooling multiple MDA reactions. The validity of using MDA was established with samples from Human African Trypanosomiasis patients. The use of MDA allows maximal use of finite DNA samples and may prove a valuable tool in studies where multiple reactions are necessary, such as population genetic analyses.
Indexed as
Identifiers
17556624PMC2067248What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.