ArticlePlant physiology2007
Involvement of a soybean ATP-binding cassette-type transporter in the secretion of genistein, a signal flavonoid in legume-Rhizobium symbiosis.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 195 citations in OpenAlex.
- Spatial Variation in Nodule Flavonoids of Soybean by Exogenous Nitrogen.Plant, cell & environment · 2026Article
- Molecular mechanisms modulating beneficial plant root-microbe interactions: What's common?Plant communications · 2026Review
- Comprehensive review of biosynthesis, plant function, metabolism and new therapeutic bioactivities of flavonoids.ADMET & DMPK · 2026Review
- Efficient, cell-type-specific production of flavonols by multiplexed CRISPR activation of a suite of metabolic enzymes.Nature communications · 2025Article
- Weighted gene co-expression network analysis - based selection of hub genes related to phenolic and volatile compounds and seed coat color in sorghum.BMC plant biology · 2025Article
- Extracellular Vesicle-Driven Crosstalk between Legume Plants and Rhizobia: The Peribacteroid Space of Symbiosomes as a Protein Trafficking Interface.Journal of proteome research · 2025Article
- Extracellular vesicles in the rhizosphere: targets to improve nutrient use efficiency of crops?Frontiers in plant science · 2025Review
- Biosynthesis and metabolic engineering of isoflavonoids in model plants and crops: a review.Frontiers in plant science · 2024Review
- Isoflavonoid metabolism in leguminous plants: an update and perspectives.Frontiers in plant science · 2024Review
- Signaling in Legume-Rhizobia Symbiosis.International journal of molecular sciences · 2023Review
- Recent advancements in multifaceted roles of flavonoids in plant-rhizomicrobiome interactions.Frontiers in plant science · 2023Review
- Progressive drought alters the root exudate metabolome and differentially activates metabolic pathways in cotton (Frontiers in plant science · 2023Article
- Constitutive overexpression of GsIMaT2 gene from wild soybean enhances rhizobia interaction and increase nodulation in soybean (Glycine max).BMC plant biology · 2022Article
- Characterization of Triterpene Saponin Glycyrrhizin Transport byPlants (Basel, Switzerland) · 2022Article
- Current perspectives on the beneficial effects of soybean isoflavones and their metabolites on plants.Food science and biotechnology · 2022Review
- Biochemistry and Molecular Basis of Intracellular Flavonoid Transport in Plants.Plants (Basel, Switzerland) · 2022Review
- Pantranscriptome combined with phenotypic quantification reveals germplasm kinship and regulation network of bract color variation inFrontiers in plant science · 2022Article
- Systemic regulation of soybean nodulation and nitrogen fixation by nitrogen via isoflavones.Frontiers in plant science · 2022Article
- A roadmap of plant membrane transporters in arbuscular mycorrhizal and legume-rhizobium symbioses.Plant physiology · 2021Review
- Identification and functional characterization of a PDR transporter in Tripterygium wilfordii Hook.f. that mediates the efflux of triptolide.Plant molecular biology · 2021Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legume plants have an ability to fix atmospheric nitrogen into nutrients via symbiosis with soil microbes. As the initial event of the symbiosis, legume plants secrete flavonoids into the rhizosphere to attract rhizobia. Secretion of flavonoids is indispensable for the establishment of symbiotic nitrogen fixation, but almost nothing is known about the membrane transport mechanism of flavonoid secretion from legume root cells. In this study, we performed biochemical analyses to characterize the transport mechanism of flavonoid secretion using soybean (Glycine max) in which genistein is a signal flavonoid. Plasma membrane vesicles prepared from soybean roots showed clear transport activity of genistein in an ATP-dependent manner. This transport activity was inhibited by sodium orthovanadate, a typical inhibitor of ATP-binding cassette (ABC) transporters, but was hardly affected by various ionophores, such as gramicidin D, nigericin, or valinomycin, suggesting involvement of an ABC transporter in the secretion of flavonoids from soybean roots. The K(m) and V(max) values of this transport were calculated to be 158 mum and 322 pmol mg protein(-1) min(-1), respectively. Competition experiments using various flavonoids of both aglycone and glucoside varieties suggested that this ABC-type transporter recognizes genistein and daidzein, another signaling compound in soybean root exudates, as well as other isoflavonoid aglycones as its substrates. Transport activity was constitutive regardless of the availability of nitrogen nutrition. This is, to our knowledge, the first biochemical characterization of the membrane transport of flavonoid secretion from roots.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.