ArticleBMC genomics2007
Directional responses following recombinant cytokine stimulation of rainbow trout (Oncorhynchus mykiss) RTS-11 macrophage cells as revealed by transcriptome profiling.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 104 citations in OpenAlex.
- Production and validation of recombinant hamster cytokines as tools for immunological studies.Molecular biology reports · 2026Article
- Spatiotemporal Dynamics of Pro-Inflammatory Mediator Expression inAnimals : an open access journal from MDPI · 2025Article
- Host-pathogen interaction unveiled by immune, oxidative stress, and cytokine expression analysis to experimental Saprolegnia parasitica infection in Nile tilapia.Scientific reports · 2023Article
- Article
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- Functional and Molecular Immune Response of Rainbow Trout (International journal of molecular sciences · 2022Article
- Gut Associated Lymphoid Tissue (GALT) primary cells and stable cell lines as predictive models for intestinal health in rainbow trout (Frontiers in immunology · 2022Article
- Lumpfish (Frontiers in immunology · 2021Article
- Oral Administration ofFrontiers in immunology · 2021Article
- NOD-like receptors mediate inflammatory lung injury during plateau hypoxia exposure.Journal of physiological anthropology · 2020Article
- Review
- The Innate Immune Response of Atlantic Salmon (Frontiers in immunology · 2020Article
- Review
- The Function of Fish Cytokines.Biology · 2016Review
- Article
- Sensors of Infection: Viral Nucleic Acid PRRs in Fish.Biology · 2015Review
- The MHC class I genes of zebrafish.Developmental and comparative immunology · 2014Review
- Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar).BMC genomics · 2013Article
- Roles of inflammatory caspases during processing of zebrafish interleukin-1β in Francisella noatunensis infection.Infection and immunity · 2012Article
- Functional identification of dendritic cells in the teleost model, rainbow trout (Oncorhynchus mykiss).PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe early stages of the immune response are regulated by key cytokines including both interleukin 1beta (IL-1beta) and interferon-gamma (IFN-gamma) which stimulate panels of responsive genes via conserved signal transduction pathways. To further our understanding of the transcriptional response to these cytokines in lower vertebrates we have utilized microarray analysis to characterize the transcriptional response to recombinant rainbow trout IL-1beta and IFN-gamma in the trout macrophage cell line RTS-11.
resultsRNA was extracted from stimulated or control cells following 6 h incubation and used to hybridize to a salmonid cDNA microarray containing 16,006 different genes. Analysis of the arrays revealed mRNA transcripts that were differentially expressed as a result of exposure to the recombinant proteins, with some responses common for both cytokines. In general the recombinant IL-1beta elicited a response where genes involved in the acute phase response were up-regulated, whilst the recombinant IFN-gamma induced strong up-regulation of genes involved in the MHC class I antigen presentation pathway. Key genes were chosen that were differentially regulated and analysed by real time PCR at additional time points, up to 48 h following stimulation. This allowed a deeper insight into the kinetics of the response to the cytokines in this cell line.
conclusionWe demonstrated that in fish both rIL-1beta and rIFN-gamma stimulated discrete panels of mRNA transcripts which indicted the cells were being directed towards different cellular functions, with IL-beta inducing genes involved in the inflammatory response, whereas IFN-gamma induced genes associated with antigen presentation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.