ArticleJournal of molecular medicine (Berlin, Germany)2007
Recombinant full-length human IgG1s targeting hormone-refractory prostate cancer.
Article in Journal of molecular medicine (Berlin, Germany), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Simultaneous Maturation of Single Chain Antibody Stability and Affinity by CHO Cell Display.Bioengineering (Basel, Switzerland) · 2022Article
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- Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166.Nature communications · 2020Article
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- Cell-type specific potent Wnt signaling blockade by bispecific antibody.Scientific reports · 2018Article
- Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content Analysis.Methods in enzymology · 2017Article
- High-content analysis of antibody phage-display library selection outputs identifies tumor selective macropinocytosis-dependent rapidly internalizing antibodies.Molecular & cellular proteomics : MCP · 2014Article
- Enhanced immunoPET of ALCAM-positive colorectal carcinoma using site-specific ⁶⁴Cu-DOTA conjugation.Protein engineering, design & selection : PEDS · 2014Article
- Selection and characterization of cell binding and internalizing phage antibodies.Archives of biochemistry and biophysics · 2012Review
- An engineered cysteine-modified diabody for imaging activated leukocyte cell adhesion molecule (ALCAM)-positive tumors.Molecular imaging and biology · 2012Article
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- Discovery of internalizing antibodies to tumor antigens from phage libraries.Methods in enzymology · 2012Article
- Article
- Beyond natural antibodies: the power of in vitro display technologies.Nature biotechnology · 2011Article
- Internalizing cancer antibodies from phage libraries selected on tumor cells and yeast-displayed tumor antigens.Journal of molecular biology · 2010Article
- Identification of internalizing human single-chain antibodies targeting brain tumor sphere cells.Molecular cancer therapeutics · 2010Article
- Human antibodies targeting cell surface antigens overexpressed by the hormone refractory metastatic prostate cancer cells: ICAM-1 is a tumor antigen that mediates prostate cancer cell invasion.Journal of molecular medicine (Berlin, Germany) · 2009Article
- Conversion of murine antibodies to human antibodies and their optimization for ovarian cancer therapy targeted to the folate receptor.Cancer immunology, immunotherapy : CII · 2009Article
- Killing cancer cells by targeted drug-carrying phage nanomedicines.BMC biotechnology · 2008Article
- Targeted drug delivery to mesothelioma cells using functionally selected internalizing human single-chain antibodies.Molecular cancer therapeutics · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have used a naive human single-chain fragment variable (scFv) library as a source of random shape repertoire to directly probe the altered surface chemistry of tumor cells. We reported previously the identification of more than 90 internalizing phage monoclonal antibodies targeting prostate cancer cells, including those that are hormone refractory. In this report, we describe the conversion of a panel of those scFvs into full-length human immunoglobulins (IgGs) and show that tumor specificity is retained. We have further shown that antibodies isolated from a naive phage display library can nevertheless be of high affinity towards target tumor cells. In addition, full-length IgGs retain the functionality of parental scFvs including the ability to rapidly enter target cells through receptor-mediated endocytosis and thereby to mediate efficient and specific intracellular payload delivery to tumor cells. We have used recombinant IgGs to immunoprecipitate target antigens and analyzed their molecular composition by mass spectrometry. We have identified one target antigen as activated leukocyte cell adhesion molecule (ALCAM)/MEMD/CD166 and have further studied tissue specificity of this internalizing ALCAM epitope by immunohistochemistry. Our study shows that cell type-specific internalizing human antibody can be readily identified from a naive phage antibody display library, characterized with regards to sequence, affinity, tissue specificity, and antigen identity, and modified genetically and chemically to generate various forms of targeted therapeutics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.