ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2007
Coupling of neuronal nitric oxide synthase to NMDA receptors via postsynaptic density-95 depends on estrogen and contributes to the central control of adult female reproduction.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Preoptic kisspeptin-nNOS-GnRH (KiNG) neuronal network regulates LH rhythmicity through activation-inhibition in mice.Nature communications · 2026Article
- GnRH and Cognition.Endocrinology · 2025Review
- Estrogen receptor-α signaling in tanycytes lies at the crossroads of fertility and metabolism.Metabolism: clinical and experimental · 2024Article
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- Nitric oxide signalling in the brain and its control of bodily functions.British journal of pharmacology · 2020Review
- DLG2 variants in patients with pubertal disorders.Genetics in medicine : official journal of the American College of Medical Genetics · 2020Article
- Effect of neuronal nitric oxide synthase serine-1412 phosphorylation on hypothalamic-pituitary-ovarian function and leptin response.Biology of reproduction · 2020Article
- Emerging Roles of Anti-Müllerian Hormone in Hypothalamic-Pituitary Function.Neuroendocrinology · 2019Review
- Neuronal nitric oxide synthase and affective disorders.IBRO reports · 2018Review
- Phenotyping of nNOS neurons in the postnatal and adult female mouse hypothalamus.The Journal of comparative neurology · 2017Article
- Late Arc/Arg3.1 expression in the basolateral amygdala is essential for persistence of newly-acquired and reactivated contextual fear memories.Scientific reports · 2016Article
- From synaptically localized to volume transmission by nitric oxide.The Journal of physiology · 2016Review
- Scaffolding protein Homer1a protects against NMDA-induced neuronal injury.Cell death & disease · 2015Article
- Activation of neuronal NMDA receptors triggers transient ATP-mediated microglial process outgrowth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Leptin-dependent neuronal NO signaling in the preoptic hypothalamus facilitates reproduction.The Journal of clinical investigation · 2014Article
- Structural plasticity: mechanisms and contribution to developmental psychiatric disorders.Frontiers in neuroanatomy · 2014Review
- Loss of autophagy in pro-opiomelanocortin neurons perturbs axon growth and causes metabolic dysregulation.Cell metabolism · 2012Article
- Bisphenol-A impairs memory and reduces dendritic spine density in adult male rats.Behavioral neuroscience · 2012Article
- Kisspeptin-GPR54 signaling in mouse NO-synthesizing neurons participates in the hypothalamic control of ovulation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Considerable research has been devoted to the understanding of how nitric oxide (NO) influences brain function. Few studies, however, have addressed how its production is physiologically regulated. Here, we report that protein-protein interactions between neuronal NO synthase (nNOS) and glutamate NMDA receptors via the scaffolding protein postsynaptic density-95 (PSD-95) in the hypothalamic preoptic region of adult female rats is sensitive to cyclic estrogen fluctuation. Coimmunoprecipitation experiments were used to assess the physical association between nNOS and NMDA receptor NR2B subunit in the preoptic region of the hypothalamus. We found that nNOS strongly interacts with NR2B at the onset of the preovulatory surge at proestrus (when estrogen levels are highest) compared with basal-stage diestrous rats. Consistently, estrogen treatment of gonadectomized female rats also increases nNOS/NR2B complex formation. Moreover, endogenous fluctuations in estrogen levels during the estrous cycle coincide with changes in the physical association of nNOS to PSD-95 and the magnitude of NO release in the preoptic region. Finally, temporary and local in vivo suppression of PSD-95 synthesis by using antisense oligodeoxynucleotides leads to inhibition of nNOS activity in the preoptic region and disrupted estrous cyclicity, a process requiring coordinated activation of neurons containing gonadotropin-releasing hormone (the neuropeptide controlling reproductive function). In conclusion, our findings identify a novel steroid-mediated molecular mechanism that enables the adult mammalian brain to control NO release under physiological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.