ArticleWorld journal of gastroenterology2007
Protein profile of human hepatocarcinoma cell line SMMC-7721: identification and functional analysis.
Article in World journal of gastroenterology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 34 citations in OpenAlex.
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- Role of a genetic variation in the microRNA-4421 binding site of ERP29 regarding risk of oropharynx cancer and prognosis.Scientific reports · 2020Article
- EGFRvIII-specific CAR-T cells produced by piggyBac transposon exhibit efficient growth suppression against hepatocellular carcinoma.International journal of medical sciences · 2020Article
- Mitochondrial inner membrane protein, Mic60/mitofilin in mammalian organ protection.Journal of cellular physiology · 2019Review
- Identification of cellular genes and pathways important for tumorigenicity of hepatocellular carcinoma cell lines by proteomic profiling.Oncotarget · 2017Article
- Identification of specific biomarkers for gastric adenocarcinoma by ITRAQ proteomic approach.Scientific reports · 2016Article
- Diagnostic and prognostic significance of serum apolipoprotein C-I in triple-negative breast cancer based on mass spectrometry.Cancer biology & therapy · 2016Article
- Diagnostic and prognostic role of serum protein peak at 6449 m/z in gastric adenocarcinoma based on mass spectrometry.British journal of cancer · 2016Article
- A novel prognostic factor for hepatocellular carcinoma: protein disulfide isomerase.The Korean journal of internal medicine · 2014Article
- Enhancement of hexokinase II inhibitor-induced apoptosis in hepatocellular carcinoma cells via augmenting ER stress and anti-angiogenesis by protein disulfide isomerase inhibition.Journal of bioenergetics and biomembranes · 2012Article
- Alpha-1 antitrypsin is a potential biomarker for hepatitis B.Virology journal · 2011Article
- Mitochondrial proteomics analysis of tumorigenic and metastatic breast cancer markers.Functional & integrative genomics · 2011Article
- Systematic identification of genes involved in divergent skeletal muscle growth rates of broiler and layer chickens.BMC genomics · 2009Article
- Comparative proteomics analysis of human gastric cancer.World journal of gastroenterology · 2008Article
- Low intensity ultrasound-induced apoptosis in human gastric carcinoma cells.World journal of gastroenterology · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to explore the new drug targets for liver cancer therapy.
methodsTotal proteins from human hepatocarcinoma cell line SMMC-7721 were separated by two-dimensional electrophoresis (2DE). The silver-stained gel was analyzed by 2DE software Image Master 2D Elite. Interesting protein spots were identified by peptide mass fingerprinting based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.
resultsWe obtained protein profile of human hepatocarcinoma cell line SMMC-7721. Among the twenty-one successfully identified proteins, mitofilin, endoplasmic reticulum protein ERp29, ubiquinol-cytochrome C reductase complex core protein I, peroxisomal enoyl CoA hydratase, peroxiredoxin-4 and probable 3-oxoacid CoA transferase 1 precursor were the six novel proteins identified in human hepatocarcinoma cells or tissues. Specific functions of the identified heat-shock proteins were analyzed in detail, and the results suggested that these proteins might promote tumorigenesis via inhibiting cell death induced by several cancer-related stresses or via inhibiting apoptosis at multiple points in the apoptotic signal pathway. Other identified chaperones and cancer-related proteins were also analyzed.
conclusionBased on the protein profile of SMMC-7721 cells, functional analysis suggests that the identified chaperones and cancer-related proteins have their own pathways to contribute to the tumorigenesis, tumor growth and metastasis of liver cancer. Furthermore, proteomic analysis is indicated to be feasible in the cancer study.
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