ArticleCurrent genetics2007
Native and modified lactate dehydrogenase expression in a fumaric acid producing isolate Rhizopus oryzae 99-880.
Article in Current genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Mucoricin is a ricin-like toxin that is critical for the pathogenesis of mucormycosis.Nature microbiology · 2021Article
- CRISPR-Cas9 induces point mutation in the mucormycosis fungus Rhizopus delemar.Fungal genetics and biology : FG & B · 2019Article
- Genetic tools for investigating Mucorales fungal pathogenesis.Current clinical microbiology reports · 2018Article
- Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species.Nature communications · 2018Article
- Phylogenetic and Phylogenomic Definition ofG3 (Bethesda, Md.) · 2018Article
- Fob1 and Fob2 Proteins Are Virulence Determinants of Rhizopus oryzae via Facilitating Iron Uptake from Ferrioxamine.PLoS pathogens · 2015Article
- Expression of Lactate Dehydrogenase in Aspergillus niger for L-Lactic Acid Production.PloS one · 2015Article
- CotH3 mediates fungal invasion of host cells during mucormycosis.The Journal of clinical investigation · 2014Article
- Metabolic engineering of Rhizopus oryzae for the production of platform chemicals.Applied microbiology and biotechnology · 2012Review
- Production of cyanophycin in Rhizopus oryzae through the expression of a cyanophycin synthetase encoding gene.Applied microbiology and biotechnology · 2012Article
- Structure, function, and phylogeny of the mating locus in the Rhizopus oryzae complex.PloS one · 2010Article
- The high affinity iron permease is a key virulence factor required for Rhizopus oryzae pathogenesis.Molecular microbiology · 2010Article
- A single dicer gene is required for efficient gene silencing associated with two classes of small antisense RNAs in Mucor circinelloides.Eukaryotic cell · 2009Article
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Authors and funding
2 authors at 2 institutions in 1 country.
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Abstract
Rhizopus oryzae is subdivided into two groups based on genetic and phenotypic differences. Type-I isolates accumulate primarily lactic acid when grown in the presence of a fermentable carbon source and contain two lactate dehydrogenase genes, ldhA and ldhB. Type-II isolates synthesize predominantly fumaric acid and only have an ldhB gene. In this study, we determined that ldhB transcript is only minimally expressed in the Type-II isolate R. oryzae 99-880. LdhB enzyme purified from gene clones isolated from the Type-I isolate R. oryzae NRRL 395 and the Type-II isolate R. oryzae 99-880 each showed reductive LDH activity (pyruvate to lactate), while no oxidative LDH activity (lactate to pyruvate) was detected in either preparation. A transformation system was then developed for the first time with R. oryzae 99-880, using a uracil auxotrophic isolate that could be complemented with an orotate phosphoribosyltransferase gene, pyrF, isolated in this study. Transformation of this Type-II isolate with the ldhA gene from R. oryzae NRRL 395 resulted in reductive LDH activity between 1.0 and 1.8 U/mg total protein. Additionally, transformed isolates grown with glucose accumulated up to 27 g lactic acid/l with a concurrent decrease in fumaric acid, ethanol, and glycerol compared with the untransformed and vector-transformed control strains.
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