ArticleProceedings of the National Academy of Sciences of the United States of America2007
Global distribution of negative cofactor 2 subunit-alpha on human promoters.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Aspergillus fumigatus Transcription Factors Involved in the Caspofungin Paradoxical Effect.mBio · 2020Article
- The negative cofactor 2 complex is a key regulator of drug resistance in Aspergillus fumigatus.Nature communications · 2020Article
- The global regulator Ncb2 escapes from the core promoter and impacts transcription in response to drug stress in Candida albicans.Scientific reports · 2017Article
- Ncb2 is involved in activated transcription of CDR1 in azole-resistant clinical isolates of Candida albicans.Eukaryotic cell · 2011Article
- Genome-wide localization analysis of a complete set of Tafs reveals a specific effect of the taf1 mutation on Taf2 occupancy and provides indirect evidence for different TFIID conformations at different promoters.Nucleic acids research · 2010Article
- Dr1 (NC2) is present at tRNA genes and represses their transcription in human cells.Nucleic acids research · 2010Article
- Basal core promoters control the equilibrium between negative cofactor 2 and preinitiation complexes in human cells.Genome biology · 2010Article
- RNA polymerase II C-terminal heptarepeat domain Ser-7 phosphorylation is established in a mediator-dependent fashion.The Journal of biological chemistry · 2010Article
- Tracking the roots of cellulase hyperproduction by the fungus Trichoderma reesei using massively parallel DNA sequencing.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The basal initiation machinery: beyond the general transcription factors.Current opinion in cell biology · 2009Review
- Active promoters and insulators are marked by the centrosomal protein 190.The EMBO journal · 2009Article
- Regulation of nuclear import and export of negative cofactor 2.The Journal of biological chemistry · 2009Article
- TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation.Molecular and cellular biology · 2009Article
- The dynamic personality of TATA-binding protein.Trends in biochemical sciences · 2009Article
- Maneuver at the transcription start site: Mot1p and NC2 navigate TFIID/TBP to specific core promoter elements.Epigenetics · 2009Article
- Human T-cell leukemia virus type 1 Tax relieves repression of proliferating cell nuclear antigen gene expression.Journal of virology · 2008Article
- TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription.Genes & development · 2008Article
- Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome.Genes & development · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Negative cofactor 2 (NC2) forms a stable complex with TATA-binding protein (TBP) on promoters in vitro. Its association with TBP prevents the binding of TFIIB and leads to inhibition of preinitiation complex formation. Here, we investigate the association of NC2 subunit-alpha with human RNA polymerase II promoter regions by using gene-specific ChIP and genome-wide promoter ChIPchip analyses. We find NC2alpha associated with a large number of human promoters, where it peaks close to the core regions. NC2 occupancy in vivo positively correlates with mRNA levels, which perhaps reflects its capacity to stabilize TBP on promoter regions. In single gene analyses, we confirm core promoter binding and in addition map the NC2 complex to enhancer proximal regions. High-occupancy histone genes display a stable NC2/TFIIB ratio during the cell cycle, which otherwise varies markedly from one gene to another. The latter is at least in part explained by an observed negative correlation of NC2 occupancy with the presence of the TFIIB recognition element in core promoter regions. Our data establish the genome-wide basis for general and gene-specific functions of NC2 in mammalian cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.