ArticleVirus genes2007
Characterisation and partial sequence analysis of two novel cypoviruses isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae).
Article in Virus genes, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- Article
- The Operophtera brumata Nucleopolyhedrovirus (OpbuNPV) Represents an Early, Divergent Lineage within Genus Alphabaculovirus.Viruses · 2017Article
- Genomes of viral isolates derived from different mosquitos species.Virus research · 2017Article
- Polyhedra structures and the evolution of the insect viruses.Journal of structural biology · 2015Article
- A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering.Scientific reports · 2015Article
- In cellulo structure determination of a novel cypovirus polyhedrin.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Genomic and biological characterization of a new cypovirus isolated from Dendrolimus punctatus.PloS one · 2014Article
- Sequence analysis of a reovirus isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae) and its parasitoid wasp Phobocampe tempestiva (Hymenoptera: Ichneumonidae).Virus research · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The complete nucleotide sequences of genomic segments S5 to S10 from Operophtera brumata cypovirus 18 (OpbuCPV18), and the complete nucleotide sequences of genomic segments S2, S5, S9 and S10 from Operophtera brumata cypovirus 19 (OpbuCPV19) have been determined. Each genome segment contained a single open reading frame (ORF). Conserved motifs 5' (AGUAAA....GUUAGCU) 3' were found at the ends of each segment of OpbuCPV18, whilst conserved motifs 5' (AACAAA....UUUGC) 3' were found at each segment terminus of OpbuCPV19. The putative proteins were compared with those of other members of the Reoviridae family. Phylogenetic analysis using the polyhedrin gene (S10) indicated that OpbuCPV18 was most closely related to Dendrolimus punctatus cypovirus 1, whilst OpbuCPV19 was most closely related to Trichoplusia ni cypovirus 15. In addition, analysis of S2, which encoded a putative RNA-dependant RNA polymerase gene, confirmed OpbuCPV19 belonged to the genus Cypovirus. Following the expression of the ORF from OpbuCPV19 S10, using a baculovirus expression vector, occlusion bodies were observed in insect cell culture. This demonstrated that segment 10 coded for the polyhedrin gene, capable of forming a polyhedral crystalline matrix.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.