ArticleNature chemical biology2007
Extension of Drosophila melanogaster life span with a GPCR peptide inhibitor.
Article in Nature chemical biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Article
- Extension of life span by down-regulation of enzymes catalyzing tryptophan conversion into kynurenine: Possible implications for mechanisms of aging.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- G protein-coupled receptors that influence lifespan of human and animal models.Biogerontology · 2022Review
- Directing evolution of novel ligands by mRNA display.Chemical Society reviews · 2021Review
- A time course transcriptomic analysis of host and injected oncogenic cells reveals new aspects ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- Identification of Neuropeptides and Their Receptors in the Ectoparasitoid,Frontiers in physiology · 2020Article
- Prospects and challenges of CRISPR/Cas genome editing for the study and control of neglected vector-borne nematode diseases.The FEBS journal · 2016Review
- Methuselah/Methuselah-like G protein-coupled receptors constitute an ancient metazoan gene family.Scientific reports · 2016Article
- De novo selection of oncogenes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Biology of ageing and role of dietary antioxidants.BioMed research international · 2014Review
- In vitro selection of proteins with desired characteristics using mRNA-display.Methods (San Diego, Calif.) · 2013Article
- Modulation of methuselah expression targeted to Drosophila insulin-producing cells extends life and enhances oxidative stress resistance.Aging cell · 2013Article
- Parasitization by Cotesia chilonis influences gene expression in fatbody and hemocytes of Chilo suppressalis.PloS one · 2013Article
- Article
- In vitro selection of a peptide antagonist of growth hormone secretagogue receptor using cDNA display.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells.PloS one · 2012Article
- The repertoire of G protein-coupled receptors in the human parasite Schistosoma mansoni and the model organism Schmidtea mediterranea.BMC genomics · 2011Article
- Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.PloS one · 2011Article
- The Drosophila G protein-coupled receptor, Methuselah, exhibits a promiscuous response to peptides.Protein science : a publication of the Protein Society · 2009Article
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6 authors.
Funding
Abstract
G protein-coupled receptors (GPCRs) mediate signaling from extracellular ligands to intracellular signal transduction proteins. Methuselah (Mth) is a class B (secretin-like) GPCR, a family typified by their large, ligand-binding, N-terminal extracellular domains. Downregulation of mth increases the life span of Drosophila melanogaster; inhibitors of Mth signaling should therefore enhance longevity. We used mRNA display selection to identify high-affinity (K(d) = 15 to 30 nM) peptide ligands that bind to the N-terminal ectodomain of Mth. The selected peptides are potent antagonists of Mth signaling, and structural studies suggest that they perturb the interface between the Mth ecto- and transmembrane domains. Flies constitutively expressing a Mth antagonist peptide have a robust life span extension, which suggests that the peptides inhibit Mth signaling in vivo. Our work thus provides new life span-extending ligands for a metazoan and a general approach for the design of modulators of this important class of GPCRs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.