ArticleNature protocols2007
Primary culture of bovine chromaffin cells.
Article in Nature protocols, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- Novel synaptobrevin-1 mutation causes fatal congenital myasthenic syndrome.Annals of clinical and translational neurology · 2017Article
- Profiles of secreted neuropeptides and catecholamines illustrate similarities and differences in response to stimulation by distinct secretagogues.Molecular and cellular neurosciences · 2015Article
- Mutant SNAP25B causes myasthenia, cortical hyperexcitability, ataxia, and intellectual disability.Neurology · 2014Article
- Imaging plasma membrane deformations with pTIRFM.Journal of visualized experiments : JoVE · 2014Article
- Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles.Nature communications · 2014Article
- Pyroglutamate-amyloid-β and glutaminyl cyclase are colocalized with amyloid-β in secretory vesicles and undergo activity-dependent, regulated secretion.Neuro-degenerative diseases · 2014Article
- Beta-amyloid peptides undergo regulated co-secretion with neuropeptide and catecholamine neurotransmitters.Peptides · 2013Article
- Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR.Frontiers in molecular neuroscience · 2013Article
- Proteomics of dense core secretory vesicles reveal distinct protein categories for secretion of neuroeffectors for cell-cell communication.Journal of proteome research · 2010Article
- Cathepsin L participates in the production of neuropeptide Y in secretory vesicles, demonstrated by protease gene knockout and expression.Journal of neurochemistry · 2008Article
- Sorting of the neuroendocrine secretory protein Secretogranin II into the regulated secretory pathway: role of N- and C-terminal alpha-helical domains.The Journal of biological chemistry · 2008Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
This protocol describes the primary culture of individual chromaffin cells derived by enzymatic digestion from the adrenal medulla of the bovine adrenal gland. Since the late 1970s, such cells have provided a useful model system to study neurotransmitter biosynthesis, storage and release in the catecholaminergic system. The protocol can be divided into three stages: isolation of cells (4-6 h), determination of viable cell numbers (approximately 30 min) and growth in culture (3-7 d). An alternative procedure is to perform studies in a continuous chromaffin (pheochromocytoma) cell line, such as PC12, although such transformed cells are typically less highly differentiated than primary cells. The bovine chromaffin cell procedure should yield approximately 10-20 million cells, suitable for several experiments over the subsequent 3-7 d. Typical experiments involve transmitter biosynthesis, vesicular storage, exocytotic release, stimulus coupling (signal transduction) toward secretion or transcription, or morphology, including ultrastructure. The total time, from adrenal gland harvest until functional experiments, is typically 4-8 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.