ArticleBiophysical journal2007
Relationship between membrane physical properties and secretory phospholipase A2 hydrolysis kinetics in S49 cells during ionophore-induced apoptosis.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- The Role of Serum Albumin and Secretory Phospholipase A2 in Sepsis.International journal of molecular sciences · 2024Article
- Molecular details of membrane fluidity changes during apoptosis and relationship to phospholipase A(2) activity.Biochimica et biophysica acta · 2013Article
- Lactadherin inhibits secretory phospholipase A2 activity on pre-apoptotic leukemia cells.PloS one · 2013Article
- Synergistic effects of secretory phospholipase A2 from the venom of Agkistrodon piscivorus piscivorus with cancer chemotherapeutic agents.BioMed research international · 2013Article
- Investigation into the role of phosphatidylserine in modifying the susceptibility of human lymphocytes to secretory phospholipase A(2) using cells deficient in the expression of scramblase.Biochimica et biophysica acta · 2012Article
- Relationship between membrane permeability and specificity of human secretory phospholipase A(2) isoforms during cell death.Biochimica et biophysica acta · 2011Article
- Imaging the early stages of phospholipase C/sphingomyelinase activity on vesicles containing coexisting ordered-disordered and gel-fluid domains.Journal of lipid research · 2011Article
- Article
- Kinetic evaluation of cell membrane hydrolysis during apoptosis by human isoforms of secretory phospholipase A2.The Journal of biological chemistry · 2010Article
- End-products diacylglycerol and ceramide modulate membrane fusion induced by a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa.Biochimica et biophysica acta · 2010Article
- The influence of membrane physical properties on microvesicle release in human erythrocytes.PMC biophysics · 2009Article
- Sequence of physical changes to the cell membrane during glucocorticoid-induced apoptosis in S49 lymphoma cells.Biophysical journal · 2009Article
- Effects of cholesterol on physical properties of human erythrocyte membranes: impact on susceptibility to hydrolysis by secretory phospholipase A2.Biophysical journal · 2008Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
During apoptosis, changes occur in lymphocyte membranes that render them susceptible to hydrolysis by secretory phospholipase A(2) (sPLA(2)). To study the relevant mechanisms, a simplified model of apoptosis using a calcium ionophore was applied. Kinetic and flow cytometry experiments provided key observations regarding ionophore treatment: the initial rate of hydrolysis was elevated at all enzyme concentrations, the total amount of reaction product was increased fourfold, and adsorption of the enzyme to the membrane surface was unaltered. Analysis of these results suggested that susceptibility during calcium-induced apoptosis is limited by availability of substrate rather than adsorption of enzyme. Fluorescence experiments identified three membrane alterations during apoptosis that might affect substrate access to the sPLA(2) active site. First, intercalation of merocyanine 540 into the membrane was improved, suggesting an increase in lipid spacing. Second, laurdan detected increased solvation of the lower headgroup region of the membrane. Third, the rate at which fluorescent lipids could be removed from the membrane by albumin was enhanced, implying greater vertical mobility of phospholipids. Thus, it is proposed that the membranes of apoptotic cells become susceptible to sPLA(2) through a reduction in lipid-neighbor interactions that facilitates migration of phospholipids into the enzyme active site.
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