ArticleBiophysical journal2007
Measurement and modeling of Ca2+ waves in isolated rabbit ventricular cardiomyocytes.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Review
- Physiology of intracellular calcium buffering.Physiological reviews · 2023Review
- Calcium Buffering in the Heart in Health and Disease.Circulation · 2019Review
- Ryanodine receptor cluster fragmentation and redistribution in persistent atrial fibrillation enhance calcium release.Cardiovascular research · 2015Article
- Inhibition of CaMKII phosphorylation of RyR2 prevents inducible ventricular arrhythmias in mice with Duchenne muscular dystrophy.Heart rhythm · 2013Article
- Facilitation of cytosolic calcium wave propagation by local calcium uptake into the sarcoplasmic reticulum in cardiac myocytes.The Journal of physiology · 2012Article
- Dynamic local changes in sarcoplasmic reticulum calcium: physiological and pathophysiological roles.Journal of molecular and cellular cardiology · 2012Review
- Differential sensitivity of Ca²+ wave and Ca²+ spark events to ruthenium red in isolated permeabilised rabbit cardiomyocytes.The Journal of physiology · 2010Article
- Modeling calcium waves in cardiac myocytes: importance of calcium diffusion.Frontiers in bioscience (Landmark edition) · 2010Article
- Intra-sarcoplasmic reticulum Ca2+ oscillations are driven by dynamic regulation of ryanodine receptor function by luminal Ca2+ in cardiomyocytes.The Journal of physiology · 2009Article
- Ryanodine receptor-mediated arrhythmias and sudden cardiac death.Pharmacology & therapeutics · 2009Review
- Mitochondrial free calcium regulation during sarcoplasmic reticulum calcium release in rat cardiac myocytes.Journal of molecular and cellular cardiology · 2009Article
- Assessment of sarcoplasmic reticulum Ca2+ depletion during spontaneous Ca2+ waves in isolated permeabilized rabbit ventricular cardiomyocytes.Biophysical journal · 2009Article
- Ca(2+)-calmodulin can activate and inactivate cardiac ryanodine receptors.British journal of pharmacology · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The time course and magnitude of the Ca(2+) fluxes underlying spontaneous Ca(2+) waves in single permeabilized ventricular cardiomyocytes were derived from confocal Fluo-5F fluorescence signals. Peak flux rates via the sarcoplasmic reticulum (SR) release channel (RyR2) and the SR Ca(2+) ATPase (SERCA) were not constant across a range of cellular [Ca(2+)] values. The Ca(2+) affinity (K(mf)) and maximum turnover rate (V(max)) of SERCA and the peak permeability of the RyR2-mediated Ca(2+) release pathway increased at higher cellular [Ca(2+)] loads. This information was used to create a computational model of the Ca(2+) wave, which predicted the time course and frequency dependence of Ca(2+) waves over a range of cellular Ca(2+) loads. Incubation of cardiomyocytes with the Ca(2+) calmodulin (CaM) kinase inhibitor autocamtide-2-related inhibitory peptide (300 nM, 30 mins) significantly reduced the frequency of the Ca(2+) waves at high Ca(2+) loads. Analysis of the Ca(2+) fluxes suggests that inhibition of CaM kinase prevented the increases in SERCA V(max) and peak RyR2 release flux observed at high cellular [Ca(2+)]. These data support the view that modification of activity of SERCA and RyR2 via a CaM kinase sensitive process occurs at higher cellular Ca(2+) loads to increase the maximum frequency of spontaneous Ca(2+) waves.
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