ArticleDevelopmental cell2007
Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network.
Article in Developmental cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 286 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
286 citing papers in PubMed, 489 citations in OpenAlex.
- PDE4DIP/Myomegalin as a Golgi-Centrosome Scaffold in Compartmentalized Signaling, Microtubule Organization, and Disease-Associated Pathway Remodeling.Cell biochemistry and function · 2026Review
- A coordinated transcriptional program controls de novo Golgi biogenesis.The EMBO journal · 2026Article
- Noncentrosomal Microtubule Assembly and Its Regulation.Cytoskeleton (Hoboken, N.J.) · 2026Review
- Patronin promotes the formation of dynamic microtubule seeds in vivo.Journal of cell science · 2026Article
- NuMA1 controls myonuclear motility in striated skeletal muscle through AMPK activity and is impaired in Duchenne muscular dystrophy.Cell death & disease · 2026Article
- SEC14L2 couples chaperone-mediated autophagy to microtubule stability by targeting Stathmin 1.Nature communications · 2026Article
- Experimental Tools and Techniques for Studying Microtubule Nucleation at the Centrosome and the Golgi Apparatus.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Insights into Microtubule Nucleation at the Golgi Apparatus.Sub-cellular biochemistry · 2026Review
- The Functions of the Golgin Family of Coiled-Coil Proteins.Sub-cellular biochemistry · 2026Review
- Golgi-Associated Protein JAKMIP2 Is Linked to the Centrosome and Performs Microtubule-Related Functions.Cells · 2025Article
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- Interplay between microtubule interactome, myonuclei mechanotransduction, and positioning in myopathies.Nucleus (Austin, Tex.) · 2025Review
- CLASP1/2 regulate immune synapse maturation in natural killer cells.Journal of leukocyte biology · 2025Article
- Branching, crosslinking, and decentralization of microtubules accelerates intracellular assembly.Biophysical journal · 2025Article
- GAS2L1 facilitates Golgi-derived microtubule growth and Golgi organization.Molecular biology of the cell · 2025Article
- Protecting centrosomes from fracturing enables efficient cell navigation.Science advances · 2025Article
- The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity.Cell reports · 2025Article
- An unexpected role of CLASP1 in radiation response and S-phase regulation of head and neck cancer cells.PloS one · 2025Article
- Organelle perturbation in Alzheimer's disease: do intracellular amyloid-ß and the fragmented Golgi mediate early intracellular neurotoxicity?Frontiers in cell and developmental biology · 2025Review
- Small GTPase ARL4C Associated with Various Cancers Affects Microtubule Nucleation.Biomedicines · 2024Article
226 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors at 8 institutions in 5 countries.
Funding
Abstract
Proper organization of microtubule arrays is essential for intracellular trafficking and cell motility. It is generally assumed that most if not all microtubules in vertebrate somatic cells are formed by the centrosome. Here we demonstrate that a large number of microtubules in untreated human cells originate from the Golgi apparatus in a centrosome-independent manner. Both centrosomal and Golgi-emanating microtubules need gamma-tubulin for nucleation. Additionally, formation of microtubules at the Golgi requires CLASPs, microtubule-binding proteins that selectively coat noncentrosomal microtubule seeds. We show that CLASPs are recruited to the trans-Golgi network (TGN) at the Golgi periphery by the TGN protein GCC185. In sharp contrast to radial centrosomal arrays, microtubules nucleated at the peripheral Golgi compartment are preferentially oriented toward the leading edge in motile cells. We propose that Golgi-emanating microtubules contribute to the asymmetric microtubule networks in polarized cells and support diverse processes including post-Golgi transport to the cell front.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.