ArticleBMC plant biology2007
Saccharomyces cerevisiae FKBP12 binds Arabidopsis thaliana TOR and its expression in plants leads to rapamycin susceptibility.
Article in BMC plant biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 137 citations in OpenAlex.
- Glucose-TOR Signaling Regulates Root Hair Elongation inPlants (Basel, Switzerland) · 2026Article
- The NRT1.1-NLP7 Nexus: An Integrative Signaling Nexus from Nitrate Sensing to Systemic Adaptation and Structure-Guided Engineering.Plants (Basel, Switzerland) · 2026Review
- Functional characterisation of Target of Rapamycin (TOR) signalling in Physcomitrella.Plant cell reports · 2026Article
- Pupylation-Based Proximity Labeling Unravels a Comprehensive Protein and Phosphoprotein Interactome of the Arabidopsis TOR Complex.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- OsFKBP12 transduces the sucrose signal from OsNIN8 to the OsTOR pathway in a loosely binding manner for cell division.iScience · 2025Article
- Target of Rapamycin Regulates Photosynthesis and Cell Growth inInternational journal of molecular sciences · 2022Article
- Target of rapamycin controls hyphal growth and pathogenicity through FoTIP4 in Fusarium oxysporum.Molecular plant pathology · 2021Article
- Visualizing protein-protein interactions in plants by rapamycin-dependent delocalization.The Plant cell · 2021Article
- Structural variations in papaya genomes.BMC genomics · 2021Article
- High-dose rapamycin exerts a temporary impact on T. reesei RUT-C30 through gene trFKBP12.Biotechnology for biofuels · 2021Article
- Article
- Shedding Light on the Dynamic Role of the "Target of Rapamycin" Kinase in the Fast-Growing CFrontiers in plant science · 2021Article
- A Rice Immunophilin Homolog, OsFKBP12, Is a Negative Regulator of Both Biotic and Abiotic Stress Responses.International journal of molecular sciences · 2020Article
- The Plant Target of Rapamycin: A Conduc TOR of Nutrition and Metabolism in Photosynthetic Organisms.Genes · 2020Review
- Target of Rapamycin (TOR) Negatively Regulates Ethylene Signals inInternational journal of molecular sciences · 2020Article
- Wheat Germination Is Dependent on Plant Target of Rapamycin Signaling.Frontiers in cell and developmental biology · 2020Article
- ABSCISIC ACID INSENSITIVE5 Interacts With RIBOSOMAL S6 KINASE2 to Mediate ABA Responses During Seedling Growth inFrontiers in plant science · 2020Article
- Host-induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould.Molecular plant pathology · 2019Article
- Chemical Screening Pipeline for Identification of Specific Plant Autophagy Modulators.Plant physiology · 2019Article
- Oligo-carrageenan kappa increases glucose, trehalose and TOR-P and subsequently stimulates the expression of genes involved in photosynthesis, and basal and secondary metabolisms in Eucalyptus globulus.BMC plant biology · 2019Article
6 more citing papers are in PubMed but not listed here.
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7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe eukaryotic TOR pathway controls translation, growth and the cell cycle in response to environmental signals such as nutrients or growth-stimulating factors. The TOR protein kinase can be inactivated by the antibiotic rapamycin following the formation of a ternary complex between TOR, rapamycin and FKBP12 proteins. The TOR protein is also found in higher plants despite the fact that they are rapamycin insensitive. Previous findings using the yeast two hybrid system suggest that the FKBP12 plant homolog is unable to form a complex with rapamycin and TOR, while the FRB domain of plant TOR is still able to bind to heterologous FKBP12 in the presence of rapamycin. The resistance to rapamycin is therefore limiting the molecular dissection of the TOR pathway in higher plants.
resultsHere we show that none of the FKBPs from the model plant Arabidopsis (AtFKBPs) is able to form a ternary complex with the FRB domain of AtTOR in the presence of rapamycin in a two hybrid system. An antibody has been raised against the AtTOR protein and binding of recombinant yeast ScFKBP12 to native Arabidopsis TOR in the presence of rapamycin was demonstrated in pull-down experiments. Transgenic lines expressing ScFKBP12 were produced and were found to display a rapamycin-dependent reduction of the primary root growth and a lowered accumulation of high molecular weight polysomes.
conclusionThese results further strengthen the idea that plant resistance to rapamycin evolved as a consequence of mutations in plant FKBP proteins. The production of rapamycin-sensitive plants through the expression of the ScFKBP12 protein illustrates the conservation of the TOR pathway in eukaryotes. Since AtTOR null mutants were found to be embryo lethal 1, transgenic ScFKBP12 plants will provide an useful tool for the post-embryonic study of plant TOR functions. This work also establish for the first time a link between TOR activity and translation in plant cells.
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