ArticlePflugers Archiv : European journal of physiology2007
The selectivity, voltage-dependence and acid sensitivity of the tandem pore potassium channel TASK-1: contributions of the pore domains.
Article in Pflugers Archiv : European journal of physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Allosteric coupling between transmembrane segment 4 and the selectivity filter of TALK1 potassium channels regulates their gating by extracellular pH.The Journal of biological chemistry · 2022Article
- Structural Insights into the Mechanisms and Pharmacology of KJournal of molecular biology · 2021Review
- KScience advances · 2020Article
- The Impact of HeterozygousJournal of the American Heart Association · 2017Article
- Much more than a leak: structure and function of K₂p-channels.Pflugers Archiv : European journal of physiology · 2015Review
- Molecular aspects of structure, gating, and physiology of pH-sensitive background K2P and Kir K+-transport channels.Physiological reviews · 2015Review
- A high-throughput functional screen identifies small molecule regulators of temperature- and mechano-sensitive K2P channels.ACS chemical biology · 2013Article
- A novel channelopathy in pulmonary arterial hypertension.The New England journal of medicine · 2013Article
- An extracellular ion pathway plays a central role in the cooperative gating of a K(2P) K+ channel by extracellular pH.The Journal of biological chemistry · 2013Article
- The small hydrophobic protein of the human respiratory syncytial virus forms pentameric ion channels.The Journal of biological chemistry · 2012Article
- External Ba2+ block of the two-pore domain potassium channel TREK-1 defines conformational transition in its selectivity filter.The Journal of biological chemistry · 2011Article
- Multiple modalities converge on a common gate to control K2P channel function.The EMBO journal · 2011Article
- Gating of a pH-sensitive K(2P) potassium channel by an electrostatic effect of basic sensor residues on the selectivity filter.PloS one · 2011Article
- Gating of two pore domain potassium channels.The Journal of physiology · 2010Review
- Gating the pore of potassium leak channels.European biophysics journal : EBJ · 2009Review
- The response of the tandem pore potassium channel TASK-3 (K(2P)9.1) to voltage: gating at the cytoplasmic mouth.The Journal of physiology · 2009Article
- A structural model for K2P potassium channels based on 23 pairs of interacting sites and continuum electrostatics.The Journal of general physiology · 2009Article
- Acid-sensitive ion channels and receptors.Handbook of experimental pharmacology · 2009Review
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
We have investigated the contribution to ionic selectivity of residues in the selectivity filter and pore helices of the P1 and P2 domains in the acid sensitive potassium channel TASK-1. We used site directed mutagenesis and electrophysiological studies, assisted by structural models built through computational methods. We have measured selectivity in channels expressed in Xenopus oocytes, using voltage clamp to measure shifts in reversal potential and current amplitudes when Rb+ or Na+ replaced extracellular K+. Both P1 and P2 contribute to selectivity, and most mutations, including mutation of residues in the triplets GYG and GFG in P1 and P2, made channels non-selective. We interpret the effects of these--and of other mutations--in terms of the way the pore is likely to be stabilised structurally. We show also that residues in the outer pore mouth contribute to selectivity in TASK-1. Mutations resulting in loss of selectivity (e.g. I94S, G95A) were associated with slowing of the response of channels to depolarisation. More important physiologically, pH sensitivity is also lost or altered by such mutations. Mutations that retained selectivity (e.g. I94L, I94V) also retained their response to acidification. It is likely that responses both to voltage and pH changes involve gating at the selectivity filter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.