ArticleTransgenic research2008
Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana.
Article in Transgenic research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- T-DNA orientation, distance between two T-DNAs, and the transformation target cells significantly impact vector backbone integration and efficiency of generating marker-free transgenic plants in a co-transformation system.The Plant journal : for cell and molecular biology · 2025Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- The pollen- and embryo-specific Arabidopsis DLL promoter bears good potential for application in marker-free Cre/loxP self-excision strategy.Plant cell reports · 2015Article
- Article
- Excision of a selectable marker gene in transgenic banana using a Cre/lox system controlled by an embryo specific promoter.Plant molecular biology · 2013Article
- Lysine racemase: a novel non-antibiotic selectable marker for plant transformation.Plant molecular biology · 2010Article
- Evaluation of seven promoters to achieve germline directed Cre-lox recombination in Arabidopsis thaliana.Plant cell reports · 2009Article
- Feasibility of the seed specific cruciferin C promoter in the self excision Cre/loxP strategy focused on generation of marker-free transgenic plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system.Plant physiology · 2007Article
- Recombinational cloning with plant gateway vectors.Plant physiology · 2007Review
- Marker-free transgenic plants through genetically programmed auto-excision.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of the CRE recombinase to catalyze excision of a DNA fragment flanked by directly repeated lox sites has been exploited to modify gene expression and proved to function well in particular case studies. However, very often variability in CRE expression and differences in efficiency of CRE-mediated recombination are observed. Here, various approaches were investigated to reproducibly obtain optimal CRE activity. CRE recombination was analyzed either by transforming the CRE T-DNA into plants containing a lox-flanked fragment or by transforming a T-DNA harboring a lox-flanked fragment into plants producing the CRE recombinase. Although somatic CRE-mediated excision of a lox-flanked fragment was obtained in all transformants, a variable amount of germline-transmitted deletions was found among different independent transformants, irrespective of the orientation of transformation. Also, the efficiency of CRE-mediated excision correlated well with the CRE mRNA level. In addition, CRE-mediated fragment excision was compared after floral dip and after root tissue transformation when transforming in a CRE-expressing background. Importantly, less CRE activity was needed to excise the lox-flanked fragment from the transferred T-DNA after root tissue transformation than after floral dip transformation. We hypothesize that this is correlated with the lower T-DNA copy number inserted during root transformation as compared to floral dip transformation.
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