ArticleBMC developmental biology2007
A set of stage-specific gene transcripts identified in EK stage X and HH stage 3 chick embryos.
Article in BMC developmental biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Article
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- Establishment of intestinal organoids from small intestine of growing cattle (12 months old).Journal of animal science and technology · 2022Article
- Identification of chicken LOC420478 as Bucky ball equivalent and potential germ plasm organizer in birds.Scientific reports · 2022Article
- Robust Three-Dimensional (3D) Expansion of Bovine Intestinal Organoids: An In Vitro Model as a Potential Alternative to an In Vivo System.Animals : an open access journal from MDPI · 2021Article
- Evaluation of Intestinal Epithelial Barrier Function in Inflammatory Bowel Diseases Using Murine Intestinal Organoids.Tissue engineering and regenerative medicine · 2020Article
- Regulatory elements and transcriptional control of chickenJournal of animal science and biotechnology · 2017Article
- Genomic analysis of Ugandan and Rwandan chicken ecotypes using a 600 k genotyping array.BMC genomics · 2016Article
- The proteomic and genomic teratogenicity elicited by valproic acid is preventable with resveratrol and α-tocopherol.PloS one · 2014Article
- Gene expression and DNA methylation status of chicken primordial germ cells.Molecular biotechnology · 2013Article
- MicroRNA-mediated posttranscriptional regulation is required for maintaining undifferentiated properties of blastoderm and primordial germ cells in chickens.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Composition and regulation of maternal and zygotic transcriptomes reflects species-specific reproductive mode.Genome biology · 2010Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe embryonic developmental process in avian species is quite different from that in mammals. The first cleavage begins 4 h after fertilization, but the first differentiation does not occur until laying of the egg (Eyal-Giladi and Kochav (EK) stage X). After 12 to 13 h of incubation (Hamburger and Hamilton (HH) stage 3), the three germ layers form and germ cell segregation in the early chick embryo are completed. Thus, to identify genes associated with early embryonic development, we compared transcript expression patterns between undifferentiated (stage X) and differentiated (HH stage 3) embryos.
resultsMicroarray analysis primarily showed 40 genes indicating the significant changes in expression levels between stage X and HH stage 3, and 80% of the genes (32/40) were differentially expressed with more than a twofold change. Among those, 72% (23/32) were relatively up-regulated at stage X compared to HH stage 3, while 28% (9/32) were relatively up-regulated at HH stage 3 compared to stage X. Verification and gene expression profiling of these GeneChip expression data were performed using quantitative RT-PCR for 32 genes at developmental four points; stage X (0 h), HH stage 3 (12 h), HH stage 6 (24 h), and HH stage 9 (30 h). Additionally, we further analyzed four genes with less than twofold expression increase at HH stage 3. As a result, we identified a set of stage-specific genes during the early chick embryo development; 21 genes were relatively up-regulated in the stage X embryo and 12 genes were relatively up-regulated in the HH stage 3 embryo based on both results of microarray and quantitative RT-PCR.
conclusionWe identified a set of genes with stage-specific expression from microarray Genechip and quantitative RT-PCR. Discovering stage-specific genes will aid in uncovering the molecular mechanisms involved the formation of the three germ layers and germ cell segregation in the early chick embryos.
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