ArticleBMC developmental biology2007
Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos.
Article in BMC developmental biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed, 158 citations in OpenAlex.
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- Interleukin-7 promotes porcine early embryogenesis in vitro and inner cell mass development through PI3K/AKT pathway after parthenogenetic activation.Scientific reports · 2025Article
- Epigenetic-related transcriptional reprogramming elucidated by identification and validation of a novel reference gene combination for RT-qPCR studies in porcine oocytes of contrasting quality.Molecular biology reports · 2024Article
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- Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages.BMC genomics · 2022Article
- Comparison of reference gene expression stability in mouse skeletal muscle via five algorithms.PeerJ · 2022Article
- Evaluating Autophagy in Preimplantation Embryos.Autophagy reports · 2022Review
- Dietary Yeast Cell Wall Improves Growth Performance and Prevents of Diarrhea of Weaned Pigs by Enhancing Gut Health and Anti-Inflammatory Immune Responses.Animals : an open access journal from MDPI · 2021Article
- Reverse transcription priming methods affect normalisation choices for gene expression levels in oocytes and early embryos.Molecular human reproduction · 2021Article
- Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis.Antioxidants (Basel, Switzerland) · 2021Article
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- Vitrification of porcine cumulus-oocyte complexes at the germinal vesicle stage does not trigger apoptosis in oocytes and early embryos, but activates anti-apoptotic Bcl-XL gene expression beyond the 4-cell stage.The Journal of reproduction and development · 2020Article
- Differentiating between the effects of heat stress and lipopolysaccharide on the porcine ovarian heat shock protein response1.Journal of animal science · 2019Article
- Oocytes, embryos and pluripotent stem cells from a biomedical perspective.Animal reproduction · 2019Article
- Identification of stable reference genes for quantitative PCR in koalas.Scientific reports · 2018Article
- A new set of reference housekeeping genes for the normalization RT-qPCR data from the intestine of piglets during weaning.PloS one · 2018Article
- Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods.BioMed research international · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the developing embryo, total RNA abundance fluctuates caused by functional RNA degradation and zygotic genome activation. These variations in the transcriptome in early development complicate the choice of good reference genes for gene expression studies by quantitative real time polymerase chain reaction.
resultsIn order to identify stably expressed genes for normalisation of quantitative data, within early stages of development, transcription levels were examined of 7 frequently used reference genes (B2M, BACT, GAPDH, H2A, PGK1, SI8, and UBC) at different stages of early porcine embryonic development (germinal vesicle, metaphase-2, 2-cell, 4-cell, early blastocyst, expanded blastocyst). Analysis of transcription profiling by geNorm software revealed that GAPDH, PGK1, S18, and UBC showed high stability in early porcine embryonic development, while transcription levels of B2M, BACT, and H2A were highly regulated.
conclusionGood reference genes that reflect total RNA content were identified in early embryonic development from oocyte to blastocyst. A selection of either GAPDH or PGK1, together with ribosomal protein S18 (S18), and UBC is proposed as reference genes, but the use of B2M, BACT, or H2A is discouraged.
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