ArticleLab on a chip2007
Cell detection and counting through cell lysate impedance spectroscopy in microfluidic devices.
Article in Lab on a chip, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 155 citations in OpenAlex.
- Enumeration of Bacteria in Suspensions Using Time Domain Reflectometry.ACS measurement science au · 2025Article
- Microfluidic Assays for CD4 T Lymphocyte Counting: A Review.Biosensors · 2025Review
- The Pre-Polarization and Concentration of Cells near Micro-Electrodes Using AC Electric Fields Enhances the Electrical Cell Lysis in a Sessile Drop.Biosensors · 2025Article
- A microfluidic chip for geoelectrical monitoring of critical zone processes.Lab on a chip · 2023Article
- Flow Cytometry: The Next Revolution.Cells · 2023Review
- A Simple Micromilled Microfluidic Impedance Cytometer with Vertical Parallel Electrodes for Cell Viability Analysis.Micromachines · 2023Article
- Ultrasensitive Detection of MCF-7 Cells with a Carbon Nanotube-Based Optoelectronic-Pulse Sensor Framework.ACS omega · 2022Article
- Detection and Analysis of Human Cells Based on Artificial Neural Network.Computational intelligence and neuroscience · 2022Article
- Towards Easy-to-Use Bacteria Sensing: Modeling and Simulation of a New Environmental Impedimetric Biosensor in Fluids.Sensors (Basel, Switzerland) · 2021Article
- Biosensors for Detecting Lymphocytes and Immunoglobulins.Biosensors · 2020Review
- Interfacing gene circuits with microelectronics through engineered population dynamics.Science advances · 2020Article
- Microscopic sensors using optical wireless integrated circuits.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Development of a multiplex fully automated assay for rapid quantification of CD4Biosensors & bioelectronics · 2019Article
- Point of Care Diagnostics for HIV in Resource Limited Settings: An Overview.Medicina (Kaunas, Lithuania) · 2018Review
- Electrochemical monitoring-on-chip (E-MoC) of HIV-infection in presence of cocaine and therapeutics.Biosensors & bioelectronics · 2016Article
- Getting into the brain: Potential of nanotechnology in the management of NeuroAIDS.Advanced drug delivery reviews · 2016Review
- Evaporation-induced stimulation of bacterial osmoregulation for electrical assessment of cell viability.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Application of Vertical Electrodes in Microfluidic Channels for Impedance Analysis.Micromachines · 2016Article
- Microfluidic differential immunocapture biochip for specific leukocyte counting.Nature protocols · 2016Article
- Integrating Cell Phone Imaging with Magnetic Levitation (i-LEV) for Label-Free Blood Analysis at the Point-of-Living.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Cell-based microfluidic devices have attracted interest for a wide range of applications. While optical cell counting and flow cytometry-type devices have been reported extensively, sensitive and efficient non-optical methods to detect and quantify cells attached over large surface areas within microdevices are generally lacking. We describe an electrical method for counting cells based on the measurement of changes in conductivity of the surrounding medium due to ions released from surface-immobilized cells within a microfluidic channel. Immobilized cells are lysed using a low conductivity, hypotonic media and the resulting change in impedance is measured using surface patterned electrodes to detect and quantify the number of cells. We found that the bulk solution conductance increases linearly with the number of isolated cells contributing to solution ion concentration. The method of cell lysate impedance spectroscopy is sensitive enough to detect 20 cells microL(-1), and offers a simple and efficient method for detecting and enumerating cells within microfluidic devices for many applications including measurement of CD4 cell counts in HIV patients in resource-limited settings. To our knowledge, this is the most sensitive approach using non-optical setups to enumerate immobilized cells. The microfluidic device, capable of isolating specific cell types from a complex bio-fluidic and quantifying cell number, can serve as a single use cartridge for a hand-held instrument to provide simple, fast and affordable cell counting in point-of-care settings.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.