ArticleMolecular biology of the cell2007
Fast turnover of L1 adhesions in neuronal growth cones involving both surface diffusion and exo/endocytosis of L1 molecules.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
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- Phospho-Regulation of Soma-to-Axon Transcytosis of Neurotrophin Receptors.Developmental cell · 2017Article
- Modulating the Tumor Microenvironment to Enhance Tumor Nanomedicine Delivery.Frontiers in pharmacology · 2017Review
- Neurite outgrowth induced by NGF or L1CAM via activation of the TrkA receptor is sustained also by the exocytosis of enlargeosomes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Local translation of TC10 is required for membrane expansion during axon outgrowth.Nature communications · 2014Article
- Lipid raft-dependent endocytosis of close homolog of adhesion molecule L1 (CHL1) promotes neuritogenesis.The Journal of biological chemistry · 2012Article
- Rabex-5 protein regulates the endocytic trafficking pathway of ubiquitinated neural cell adhesion molecule L1.The Journal of biological chemistry · 2012Article
- Review
- Growth cone travel in space and time: the cellular ensemble of cytoskeleton, adhesion, and membrane.Neuron · 2012Review
- Activation of ezrin/radixin/moesin mediates attractive growth cone guidance through regulation of growth cone actin and adhesion receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Article
- Ethanol causes the redistribution of L1 cell adhesion molecule in lipid rafts.Journal of neurochemistry · 2011Article
- Polarized targeting of L1-CAM regulates axonal and dendritic bundling in vitro.The European journal of neuroscience · 2010Article
- Trafficking guidance receptors.Cold Spring Harbor perspectives in biology · 2010Review
- Role of the cytoplasmic domain of the L1 cell adhesion molecule in brain development.The Journal of comparative neurology · 2010Article
- The TC10-Exo70 complex is essential for membrane expansion and axonal specification in developing neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Quantifying neurite growth mediated by interactions among secretory vesicles, microtubules, and actin networks.Biophysical journal · 2009Article
- Protein synthesis in distal axons is not required for growth cone responses to guidance cues.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- A molecular clutch between the actin flow and N-cadherin adhesions drives growth cone migration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the interplay between surface trafficking and binding dynamics of the immunoglobulin cell adhesion molecule L1 at neuronal growth cones. Primary neurons were transfected with L1 constructs bearing thrombin-cleavable green fluorescent protein (GFP), allowing visualization of newly exocytosed L1 or labeling of membrane L1 molecules by Quantum dots. Intracellular L1-GFP vesicles showed preferential centrifugal motion, whereas surface L1-GFP diffused randomly, revealing two pathways to address L1 to adhesive sites. We triggered L1 adhesions using microspheres coated with L1-Fc protein or anti-L1 antibodies, manipulated by optical tweezers. Microspheres coupled to the actin retrograde flow at the growth cone periphery while recruiting L1-GFP molecules, of which 50% relied on exocytosis. Fluorescence recovery after photobleaching experiments revealed a rapid recycling of L1-GFP molecules at L1-Fc (but not anti-L1) bead contacts, attributed to a high lability of L1-L1 bonds at equilibrium. L1-GFP molecules truncated in the intracellular tail as well as neuronal cell adhesion molecules (NrCAMs) missing the clathrin adaptor binding sequence showed both little internalization and reduced turnover rates, indicating a role of endocytosis in the recycling of mature L1 contacts at the base of the growth cone. Thus, unlike for other molecules such as NrCAM or N-cadherin, diffusion/trapping and exo/endocytosis events cooperate to allow the fast renewal of L1 adhesions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.