ArticleJournal of clinical microbiology2007
New variable-number tandem-repeat markers for typing Mycobacterium avium subsp. paratuberculosis and M. avium strains: comparison with IS900 and IS1245 restriction fragment length polymorphism typing.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
89 citing papers in PubMed, 1 synthesis or guideline pooled it, 191 citations in OpenAlex.
- Mycobacterium avium subsp. paratuberculosis (MAP) molecular diversity in cattle, sheep, and goats from Latin America and the Caribbean: a systematic review.Tropical animal health and production · 2021Pooled it
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- First whole-genome sequence of Mycobacterium avium subsp. silvaticum isolated from a diseased Egyptian goose (Alopochen aegyptiaca).BMC genomics · 2025Article
- New mini-satellite tandem repeat markers for strain typing of Mycobacterium avium subsp. paratuberculosis.Molecular biology reports · 2025Article
- First report ofJournal of veterinary research · 2025Article
- High Molecular Diversity ofInternational journal of molecular sciences · 2025Article
- Detection of a Mixed-Strain Infection with Drug- and Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Comparative genomics of Mycobacterium avium subsp. hominissuis strains within a group of captive lowland tapirs.PloS one · 2025Article
- Paratuberculosis in South American camelids: two independent cases in alpacas in Germany.BMC veterinary research · 2024Article
- Molecular Diversity ofAnimals : an open access journal from MDPI · 2023Article
- InvestigatingMicrobiology spectrum · 2023Article
- Mycobacterial Interspersed Repeat Unit-Variable Number Tandem Repeat Typing ofPathogens (Basel, Switzerland) · 2023Article
- Genomic epidemiology ofFrontiers in genetics · 2023Article
- Mycobacteriosis in slaughter pigs from South Africa from 1991 to 2002:Frontiers in microbiology · 2023Article
- Strain diversity inFrontiers in veterinary science · 2023Article
- Phylogenetic and Multiple-Locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina.Veterinary research communications · 2022Article
- Mycobacteriosis in Various Pet and Wild Birds from Germany: Pathological Findings, Coinfections, and Characterization of Causative Mycobacteria.Microbiology spectrum · 2022Article
- MIRU-VNTR Typing of Atypical Mycobacteria Isolated from the Lymph Nodes of Slaughtered Pigs from Poland.Pathogens (Basel, Switzerland) · 2022Article
- Association between the severity of histopathological lesions andFrontiers in veterinary science · 2022Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium avium subsp. paratuberculosis, the etiological agent of paratuberculosis, affects a wide range of domestic ruminants and has been suggested to be involved in Crohn's disease in humans. Most available methods for identifying and differentiating strains of this difficult species are technically demanding and have limited discriminatory power. Here, we report the identification of novel PCR-based typing markers consisting of variable-number tandem repeats (VNTRs) of genetic elements called mycobacterial interspersed repetitive units (MIRUs). Eight markers were applied to 183 M. avium subsp. paratuberculosis isolates from bovine, caprine, ovine, cervine, leporine, and human origins from 10 different countries and to 82 human isolates of the closely related species M. avium from France. Among the M. avium subsp. paratuberculosis isolates, 21 patterns were found by MIRU-VNTR typing, with a discriminatory index of 0.751. The predominant R01 IS900 restriction fragment length polymorphism type, comprising 131 isolates, was divided into 15 MIRU-VNTR types. Among the 82 M. avium isolates, the eight MIRU-VNTR loci distinguished 30 types, none of which was shared by M. avium subsp. paratuberculosis isolates, resulting in a discriminatory index of 0.889. Our results suggest that MIRU-VNTR typing is a fast typing method that, in combination with other methods, might prove to be optimal for PCR-based molecular epidemiological studies of M. avium/M. avium subsp. paratuberculosis pathogens. In addition, presumably identical M. avium subsp. paratuberculosis 316F vaccine strains originating from the Weybridge laboratory and from different commercial batches from Mérial actually differed by one or both typing methods. These results indicate a substantial degree of genetic drift among different vaccine preparations, which has important implications for prophylactic approaches.
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