ArticleJournal of clinical microbiology2007
High-throughput, sensitive, and accurate multiplex PCR-microsphere flow cytometry system for large-scale comprehensive detection of respiratory viruses.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00114881 (Urban Environment and Childhood Asthma), which is not on this map. Cited by 138 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Urban Environment and Childhood Asthma (URECA)
Who cites it
138 citing papers in PubMed, 224 citations in OpenAlex.
- Meditation or exercise for preventing acute respiratory infection (MEPARI-2): A randomized controlled trial.PloS one · 2018Trial
- Assessment of wheezing frequency and viral etiology on childhood and adolescent asthma risk.The Journal of allergy and clinical immunology · 2017Trial
- Comparison of risk factors for viral and nonviral asthma exacerbations.The Journal of allergy and clinical immunology · 2015Trial
- Association of interleukin-8 and neutrophils with nasal symptom severity during acute respiratory infection.Journal of medical virology · 2015Trial
- Detection of pathogenic bacteria during rhinovirus infection is associated with increased respiratory symptoms and asthma exacerbations.The Journal of allergy and clinical immunology · 2014Trial
- Randomized trial of omalizumab (anti-IgE) for asthma in inner-city children.The New England journal of medicine · 2011Trial
- Effects of montelukast on patients with asthma after experimental inoculation with human rhinovirus 16.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2011Trial
- Meditation or exercise for preventing acute respiratory infection: a randomized controlled trial.Annals of family medicineTrial
- Improving the Fidelity of Replication of a Six-Letter DNA Alphabet.ACS chemical biology · 2025Article
- The Association Between Respiratory Viruses and Asthma Exacerbation in Children Visiting Pediatric Emergency Department: A Retrospective Cohort Study.Journal of clinical medicine · 2025Article
- Cancer cell target discovery: comparing laboratory evolution of expanded DNA six-nucleotide alphabets with standard four-nucleotide alphabets.Nucleic acids research · 2025Article
- Viral and non-viral episodes of wheezing in early life and the development of asthma and respiratory phenotypes among urban children.Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2024Article
- Review of flow cytometry as a tool for cell and gene therapy.Cytotherapy · 2024Review
- Diagnosis of asthmatic pneumonia in children by lung ultrasound vs. chest X-ray: an updated systematic review and meta-analysis.Postepy dermatologii i alergologii · 2023Article
- Evaluation Study of xMAP TIER Assay on a Microsphere-Based Platform for Detecting First-Line Anti-Tuberculosis Drug Resistance.International journal of environmental research and public health · 2022Article
- The Impact of Cocirculating Pathogens on Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)/Coronavirus Disease 2019 Surveillance: How Concurrent Epidemics May Introduce Bias and Decrease the Observed SARS-CoV-2 Percentage Positivity.The Journal of infectious diseases · 2022Article
- Design and Evaluation of Multiplex One-Step Reverse Transcription PCR-Dipstick Chromatography Method for the Analysis of Seven Respiratory Pathogens.Current microbiology · 2021Article
- Machine Learning, COVID-19 (2019-nCoV), and multi-OMICS.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- Respiratory health, allergies, and the farm environment: design, methods and enrollment in the observational Wisconsin Infant Study Cohort (WISC): a research proposal.BMC research notes · 2019Article
- Viral respiratory infections and the oropharyngeal bacterial microbiota in acutely wheezing children.PloS one · 2019Article
78 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Human respiratory viruses are a diverse group of pathogens composed of hundreds of virus strains, and this presents a major challenge for diagnostic laboratories. To efficiently detect numerous viruses in a large epidemiologic study, we developed a fast, multitarget, sensitive, and specific assay named the Respiratory MultiCode-PLx Assay (RMA). The RMA utilizes improved multiplex PCR chemistry (EraGen MultiCode-PLx technology) coupled with high-throughput microsphere flow cytometry (Luminex). Eighteen sets of virus-specific multiplex PCR primers were developed based on the conserved sequences of all available respiratory-virus sequences for eight distinct groups: human rhinovirus (HRV), respiratory syncytial virus (RSV), parainfluenza virus (PIV), influenza virus (InfV), metapneumovirus, adenovirus (Ad), coronavirus, and enterovirus. Each primer set detected 20 cDNA copies of the intended target per sample and had no reaction with 60,000 copies of human genomic DNA. The accuracy and sensitivity of the RMA for detecting respiratory viruses in human samples were tested with two sets of clinical specimens. First, 101 nasal-wash specimens that were positive for HRV, RSV, InfV, PIV, or Ad by traditional techniques were reanalyzed by RMA, and all target viruses were detected with an overall sensitivity of 94% and specificity of 99%. Second, 103 nasal-wash samples from 5-year-old children with asthma and respiratory symptoms were analyzed; RMA detected viruses in 74 specimens (71.8%) compared to only 24 (23.3%) by traditional culture and immunofluorescent-staining techniques. These results show that RMA is an accurate, sensitive, and practical test for respiratory-virus infections.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.