ArticleJournal of virology2007
A mutation on influenza C virus M1 protein affects virion morphology by altering the membrane affinity of the protein.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Cytoplasmic domain of CM2 is involved in the replication of influenza C virus.The Journal of general virology · 2025Article
- Simultaneous Packaging of Two Different RNA Segments into an Influenza C Virus-like Particle Occurs Inefficiently.Viruses · 2025Article
- Temporal and Gene Reassortment Analysis of Influenza C Virus Outbreaks in Hong Kong, SAR, China.Journal of virology · 2022Article
- Host Range, Biology, and Species Specificity of Seven-Segmented Influenza Viruses-A Comparative Review on Influenza C and D.Pathogens (Basel, Switzerland) · 2021Review
- Identification of One Critical Amino Acid Residue of the Nucleoprotein as a Determinant forJournal of virology · 2021Article
- Influenza Reverse Genetics-Historical Perspective.Cold Spring Harbor perspectives in medicine · 2021Review
- In situ structure and organization of the influenza C virus surface glycoprotein.Nature communications · 2021Article
- Establishment of a Reverse Genetics System for Influenza D Virus.Journal of virology · 2020Article
- Unique Directional Motility of Influenza C Virus Controlled by Its Filamentous Morphology and Short-Range Motions.Journal of virology · 2018Article
- Effect of Phosphorylation of CM2 Protein on Influenza C Virus Replication.Journal of virology · 2017Article
- The Matrix protein M1 from influenza C virus induces tubular membrane invaginations in an in vitro cell membrane model.Scientific reports · 2017Article
- Genetic Lineage and Reassortment of Influenza C Viruses Circulating between 1947 and 2014.Journal of virology · 2016Article
- Filamentous influenza viruses.The Journal of general virology · 2016Review
- Hemagglutinin-esterase-fusion (HEF) protein of influenza C virus.Protein & cell · 2016Review
- A crucial role of N-terminal domain of influenza A virus M1 protein in interaction with swine importin α1 protein.Virus genes · 2014Article
- RNA virus reverse genetics and vaccine design.Viruses · 2014Review
- Many ways to make an influenza virus--review of influenza virus reverse genetics methods.Influenza and other respiratory viruses · 2013Review
- Effect of cysteine mutations in the extracellular domain of CM2 on the influenza C virus replication.PloS one · 2013Article
- Role of the CM2 protein in the influenza C virus replication cycle.Journal of virology · 2011Article
- A seven plasmid-based system for the rescue of influenza C virus.Journal of molecular and genetic medicine : an international journal of biomedical research · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse genetics has been documented for influenza A, B, and Thogoto viruses belonging to the family Orthomyxoviridae. We report here the reverse genetics of influenza C virus, another member of this family. The seven viral RNA (vRNA) segments of C/Ann Arbor/1/50 were expressed in 293T cells from cloned cDNAs, together with nine influenza C virus proteins. At 48 h posttransfection, the infectious titer of the culture supernatant was determined to be 2.51 x 10(3) 50% egg infectious doses/ml, which is lower than the number of influenza C virus-like particles (VLPs) (10(6)/ml) generated using the same system. By generating influenza C VLPs containing a given vRNA segment, we showed that each of the vRNA segments was similarly synthesized in the plasmid-transfected cells but that some segments were less efficiently incorporated into the VLPs. This finding leads us to speculate that the differences in incorporation efficiency into VLPs between segments might be a reason for the inefficient production of infectious viruses. Second, we generated a mutant recombinant virus, rMG96A, which possesses an Ala-->Thr mutation at residue 24 of the M1 protein, a substitution demonstrated to be involved in the morphology (filamentous or spherical) of the influenza C VLPs. As expected, rMG96A exhibited a spherical morphology, whereas recombinant wild-type of C/Ann Arbor/1/50, rWT, exhibited a mainly filamentous morphology. Membrane flotation analysis of the cells infected with rWT or rMG96A revealed a difference in the ratio of membrane-associated M1 proteins, suggesting that the affinity of M1 protein to the cell membrane is a determinant for virion morphology.
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