ArticleNucleic acids research2007
Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- I-Block: a simple Escherichia coli-based assay for studying sequence-specific DNA binding of proteins.Nucleic acids research · 2020Article
- In vitro Fab display: a cell-free system for IgG discovery.Protein engineering, design & selection : PEDS · 2014Article
- Determining the specificities of TALENs, Cas9, and other genome-editing enzymes.Methods in enzymology · 2014Review
- Evaluation of novel design strategies for developing zinc finger nucleases tools for treating human diseases.Biotechnology research international · 2014Article
- Seamless genome editing in human pluripotent stem cells using custom endonuclease-based gene targeting and the piggyBac transposon.Nature protocols · 2013Article
- Modeling the specificity of protein-DNA interactions.Quantitative biology (Beijing, China) · 2013Article
- Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection.Nature methods · 2011Article
- Genome engineering with zinc-finger nucleases.Genetics · 2011Review
- Sequence specificity is obtained from the majority of modular C2H2 zinc-finger arrays.Nucleic acids research · 2011Article
- Experimental strategies for studying transcription factor-DNA binding specificities.Briefings in functional genomics · 2010Review
- Modular system for the construction of zinc-finger libraries and proteins.Nature protocols · 2010Article
- Bind-n-Seq: high-throughput analysis of in vitro protein-DNA interactions using massively parallel sequencing.Nucleic acids research · 2009Article
- High-resolution profiling of homing endonuclease binding and catalytic specificity using yeast surface display.Nucleic acids research · 2009Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The C2H2 zinc finger is the most commonly utilized framework for engineering DNA-binding domains with novel specificities. Many different selection strategies have been developed to identify individual fingers that possess a particular DNA-binding specificity from a randomized library. In these experiments, each finger is selected in the context of a constant finger framework that ensures the identification of clones with a desired specificity by properly positioning the randomized finger on the DNA template. Following a successful selection, multiple zinc-finger clones are typically recovered that share similarities in the sequences of their DNA-recognition helices. In principle, each of the clones isolated from a selection is a candidate for assembly into a larger multi-finger protein, but to date a high-throughput method for identifying the most specific candidates for incorporation into a final multi-finger protein has not been available. Here we describe the development of a specificity profiling system that facilitates rapid and inexpensive characterization of engineered zinc-finger modules. Moreover, we demonstrate that specificity data collected using this system can be employed to rationally design zinc fingers with improved DNA-binding specificities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.