ArticleThe Journal of cell biology2007
Essential role of PI3-kinase and phospholipase A2 in Dictyostelium discoideum chemotaxis.
Article in The Journal of cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed, 113 citations in OpenAlex.
- Global profiling of polyketide synthases in facultative multicellular eukaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- From Obscurity to Prominence: IPMK's Expanding Role in Cellular Signaling, Physiology, and Disease.Biomolecules · 2025Review
- Two endogenousProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Persistent pseudopod splitting is an effective chemotaxis strategy in shallow gradients.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A phosphatidylinositol phosphate kinase inhibits Ras activation and regulates chemorepulsion in Dictyostelium discoideum.Journal of cell science · 2023Article
- Dynamics of Actin Cytoskeleton and Their Signaling Pathways during Cellular Wound Repair.Cells · 2022Article
- Spatiotemporal dynamics of membrane surface charge regulates cell polarity and migration.Nature cell biology · 2022Article
- The principles of directed cell migration.Nature reviews. Molecular cell biology · 2021Review
- Article
- A module for Rac temporal signal integration revealed with optogenetics.The Journal of cell biology · 2017Article
- Signaling Pathways Controlling Microglia Chemotaxis.Molecules and cells · 2017Review
- A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration.Nature communications · 2015Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- How to understand and outwit adaptation.Developmental cell · 2014Article
- Excitable signal transduction induces both spontaneous and directional cell asymmetries in the phosphatidylinositol lipid signaling system for eukaryotic chemotaxis.Biophysical journal · 2014Article
- Daydreamer, a Ras effector and GSK-3 substrate, is important for directional sensing and cell motility.Molecular biology of the cell · 2013Article
- Distinct cell shapes determine accurate chemotaxis.Scientific reports · 2013Article
- Delineating the core regulatory elements crucial for directed cell migration by examining folic-acid-mediated responses.Journal of cell science · 2013Article
- A matricellular protein and EGF-like repeat signalling in the social amoebozoan Dictyostelium discoideum.Cellular and molecular life sciences : CMLS · 2012Review
- Regulation of actin cytoskeleton by Rap1 binding to RacGEF1.Molecules and cells · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemotaxis toward different cyclic adenosine monophosphate (cAMP) concentrations was tested in Dictyostelium discoideum cell lines with deletion of specific genes together with drugs to inhibit one or all combinations of the second-messenger systems PI3-kinase, phospholipase C (PLC), phospholipase A2 (PLA2), and cytosolic Ca(2+). The results show that inhibition of either PI3-kinase or PLA2 inhibits chemotaxis in shallow cAMP gradients, whereas both enzymes must be inhibited to prevent chemotaxis in steep cAMP gradients, suggesting that PI3-kinase and PLA2 are two redundant mediators of chemotaxis. Mutant cells lacking PLC activity have normal chemotaxis; however, additional inhibition of PLA2 completely blocks chemotaxis, whereas inhibition of PI3-kinase has no effect, suggesting that all chemotaxis in plc-null cells is mediated by PLA2. Cells with deletion of the IP(3) receptor have the opposite phenotype: chemotaxis is completely dependent on PI3-kinase and insensitive to PLA2 inhibitors. This suggest that PI3-kinase-mediated chemotaxis is regulated by PLC, probably through controlling PIP(2) levels and phosphatase and tensin homologue (PTEN) activity, whereas chemotaxis mediated by PLA2 appears to be controlled by intracellular Ca(2+).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.