ArticlePlant molecular biology2007
Developmental defects and seedling lethality in apyrase AtAPY1 and AtAPY2 double knockout mutants.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- Apyrase in horticultural crops: insights into growth, stress adaptation and quality regulation.Molecular horticulture · 2025Review
- Extracellular ATP: an emerging multifaceted regulator of plant fitness.Plant biotechnology journal · 2025Review
- Genome-Wide Analysis of Soybean Apyrase Gene Family and Functional Characterization of GmAPY1-4 Responses to Aluminum Stress.International journal of molecular sciences · 2025Article
- Advances in plant male sterility for hybrid seed production: an overview of conditional nuclear male sterile lines and biotechnology-based male sterile systems.Frontiers in plant science · 2025Review
- Growth regulation by apyrases: Insights from altering their expression level in different organisms.Plant physiology · 2024Review
- APYRASE1/2 mediate red light-induced de-etiolation growth in Arabidopsis seedlings.Plant physiology · 2022Article
- Recruitment of an ancient branching program to suppress carpel development in maize flowers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Transcriptomic and Proteomic Insights intoPlant physiology · 2020Article
- Extracellular ATP promoted pollen germination and tube growth of Nicotiana tabacum through promoting KPlant reproduction · 2018Article
- Role of CaInternational journal of molecular sciences · 2018Review
- Heterotrimeric G Protein-Regulated CaFrontiers in plant science · 2017Article
- HvEXPB7, a novel β-expansin gene revealed by the root hair transcriptome of Tibetan wild barley, improves root hair growth under drought stress.Journal of experimental botany · 2015Article
- Article
- The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity.Frontiers in plant science · 2015Article
- Role for apyrases in polar auxin transport in Arabidopsis.Plant physiology · 2012Article
- The Arabidopsis apyrase AtAPY1 is localized in the Golgi instead of the extracellular space.BMC plant biology · 2012Article
- Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis.Plant physiology · 2012Article
- Extracellular nucleotides and apyrases regulate stomatal aperture in Arabidopsis.Plant physiology · 2011Article
- Equilibrative nucleoside transporter 1 (ENT1) is critical for pollen germination and vegetative growth in Arabidopsis.Journal of experimental botany · 2011Article
- Poplar maintains zinc homeostasis with heavy metal genes HMA4 and PCS1.Journal of experimental botany · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously it was shown that the Arabidopsis apyrase genes AtAPY1 and AtAPY2 are crucial for male fertility because mutant pollen (apy1-1; apy2-1) with T-DNA insertions in both genes could not germinate (Steinebrunner et al. (2003) Plant Physiol. 131: 1638-1647). In this study, pollen germination was restored and apyrase T-DNA double knockouts (DKO) apy1-1/apy1-1; apy2-1/apy2-1 were generated by complementation with AtAPY2 under the control of a pollen-specific promoter. The DKO phenotype displayed developmental defects including the lack of functional root and shoot meristems. In cotyledons, morphogenetic and patterning abnormalities were apparent, e.g., unlobed pavement cells and stomatal clusters. Another set of lines was created which carried either AtAPY1 or AtAPY2 under a dexamethasone-(DEX)-inducible promoter as an additional transgene to the pollen-specific gene construct. Application of DEX did not reverse the DKO phenotype to wild-type, but some inducible lines exhibited less severe defects even in the absence of the inducer, probably due to some background expression. However, even these DKO mutants were seedling-lethal and shared other defects regarding cell division, cell expansion and stomatal patterning. Taken together, the defects in the DKO mutants demonstrate that AtAPY1 and AtAPY2 are essential for normal plant development.
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