ArticleJournal of anatomy2007
microMRI-HREM pipeline for high-throughput, high-resolution phenotyping of murine embryos.
Article in Journal of anatomy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Selective right heart valve remodelling in a mouse model of carcinoid disease revealed by high-resolution episcopic microscopy.Scientific reports · 2025Article
- Review
- A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System.International journal of molecular sciences · 2021Review
- Cardiac multi-scale investigation of the right and left ventricleCardiovascular diagnosis and therapy · 2020Review
- X-ray-Based 3D Virtual Histology-Adding the Next Dimension to Histological Analysis.Molecular imaging and biology · 2018Review
- High-resolution Imaging of Myeloperoxidase Activity Sensors in Human Cerebrovascular Disease.Scientific reports · 2018Article
- High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials.Journal of visualized experiments : JoVE · 2017Article
- Transgenerational cardiology: One way to a baby's heart is through the mother.Molecular and cellular endocrinology · 2016Review
- Diffusion microscopic MRI of the mouse embryo: Protocol and practical implementation in the splotch mouse model.Magnetic resonance in medicine · 2015Article
- Imaging the zebrafish dentition: from traditional approaches to emerging technologies.Zebrafish · 2015Review
- Phenotyping structural abnormalities in mouse embryos using high-resolution episcopic microscopy.Disease models & mechanisms · 2014Article
- Article
- Article
- 3-dimensional imaging modalities for phenotyping genetically engineered mice.Veterinary pathology · 2012Review
- High-throughput analysis of mouse embryos by magnetic resonance imaging.Cold Spring Harbor protocols · 2012Article
- Imaging heart development using high-resolution episcopic microscopy.Current opinion in genetics & development · 2011Review
- Data ontology and an information system realization for web-based management of image measurements.Frontiers in neuroinformatics · 2011Article
- Rapid 3D phenotyping of cardiovascular development in mouse embryos by micro-CT with iodine staining.Circulation. Cardiovascular imaging · 2010Article
- Micro-computed tomography-based phenotypic approaches in embryology: procedural artifacts on assessments of embryonic craniofacial growth and development.BMC developmental biology · 2010Article
- Episcopic 3D Imaging Methods: Tools for Researching Gene Function.Current genomics · 2008Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Rapid and precise phenotyping analysis of large numbers of wild-type and mutant mouse embryos is essential for characterizing the genetic and epigenetic factors regulating embryogenesis. We present a novel methodology that permits precise high-throughput screening of the phenotype of embryos with both targeted and randomly generated mutations. To demonstrate the potential of this methodology we show embryo phenotyping results produced in a large-scale ENU-mutagenesis study. In essence this represents an analysis pipeline, which starts with simultaneous micro-magentic resonance imaging (microMRI) screening (voxel size: 25.4 x 25.4 x 24.4 microm) of 32 embryos in one run. Embryos with an indistinct phenotype are then cut into parts and suspect organs and structures are analysed with HREM (high-resolution episcopic microscopy). HREM is an imaging technique that employs 'positive' eosin staining and episcopic imaging for generating three-dimensional (3D) high-resolution (voxel size: 1.07 x 1.07 x 2 microm) digital data of near histological contrast and quality. The results show that our method guarantees the rapid availability of comprehensive phenotype information for high numbers of embryos in, if necessary, histological quality and detail. The combination of high-throughput microMRI with HREM provides an alternative screening pipeline with advantages over existing 3D phenotype screening methods as well as traditional histology. Thus, the microMRI-HREM phenotype analysis pipeline recommends itself as a routine tool for analysing the phenotype of transgenic and mutant embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.