ArticleJournal of in vitro fertilization and embryo transfer : IVF1991
Reliability of gender determination using the polymerase chain reaction (PCR) for single cells.
Article in Journal of in vitro fertilization and embryo transfer : IVF, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Gender selection: cultural and religious perspectives.Journal of assisted reproduction and genetics · 2002Review
- An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method.Journal of assisted reproduction and genetics · 1999Article
- Preimplantation diagnosis of inherited disease by embryo biopsy: an update of the world figures.Journal of assisted reproduction and genetics · 1996Article
- Identifying the sex of human preimplantation embryos in X-linked disease: amplification efficiency of a Y-specific alphoid repeat from single blastomeres with two lysis protocols.Journal of assisted reproduction and genetics · 1996Article
- Review: preimplantation diagnosis of inherited disease.Journal of inherited metabolic disease · 1996Review
- Optimal polymerase chain reaction amplification for preimplantation diagnosis in cystic fibrosis (delta F508)BMJ (Clinical research ed.) · 1995Article
- Chromosome abnormalities in human arrested preimplantation embryos: a multiple-probe FISH study.American journal of human genetics · 1994Article
- Reliability of polymerase chain reaction (PCR) analysis of single cells for preimplantation genetic diagnosis.Journal of assisted reproduction and genetics · 1994Article
- Preimplantation diagnosis.The Western journal of medicine · 1993Review
- Sex determination in single mouse blastomeres by polymerase chain reaction.Journal of assisted reproduction and genetics · 1993Article
- A fast and efficient method for simultaneous X and Y in situ hybridization of human blastomeres.Journal of assisted reproduction and genetics · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contamination with extraneous DNA sequences is a frequent problem when performing PCR analysis of single cells. This report describes our experience with eliminating contaminating DNA sequences from PCR reagents for the purposes of gender identification. We have used amplification of Y-specific sequences to identify the gender of single human amniocytes. Female cells consistently showed no Y-specific bands but only 80% of male cells showed the expected intense Y-specific band. This phenomenon could lead to incorrect gender identification of single cells. We developed a technique of simultaneous amplification of X- and Y-specific sequences to prevent misdiagnosis because of failed PCR, which allows accurate preimplantation gender determination for women at risk for conceiving children with X-linked genetic diseases. We analyzed the gender of 141 consecutive single cells in a blinded manner without a single incorrect gender assignment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.