ArticleGenome biology2007
Optimization and clinical validation of a pathogen detection microarray.
Article in Genome biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Broad-spectrum molecular detection of zoonotic agents in wild micromammals: a nationwide surveillance study in the Republic of Korea.Parasites & vectors · 2026Article
- Multiplex detection and identification of viral, bacterial, and protozoan pathogens in human blood and plasma using an expanded high-density resequencing microarray platform.Frontiers in molecular biosciences · 2024Article
- An Algorithm for the Selection of Probes for Specific Detection of Human Disease Pathogens Using the DNA Microarray Technology.Sovremennye tekhnologii v meditsine · 2022Article
- DNA Microarray Platform for Detection and Surveillance of Viruses Transmitted by Small Mammals and Arthropods.PLoS neglected tropical diseases · 2016Article
- [Identification of bocavirus infection in a young child with pneumonia using a pan-microbial microarray].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2016Article
- Hepatitis Delta Virus Detected in Salivary Glands of Sjögren's Syndrome Patients and Recapitulates a Sjögren's Syndrome-Like PhenotypePathogens & immunity · 2016Article
- Viral diagnosis in Indian livestock using customized microarray chips.Bioinformation · 2015Article
- LNA-modified isothermal oligonucleotide microarray for differentiating bacilli of similar origin.Journal of biosciences · 2014Article
- Article
- Pathogen chip for respiratory tract infections.Journal of clinical microbiology · 2013Article
- Article
- Recent advances in diagnosis, prevention, and treatment of human respiratory syncytial virus.Advances in virology · 2013Review
- Emerging view of the human virome.Translational research : the journal of laboratory and clinical medicine · 2012Review
- Modeling formamide denaturation of probe-target hybrids for improved microarray probe design in microbial diagnostics.PloS one · 2012Article
- Rapid detection of urinary tract infections using isotachophoresis and molecular beacons.Analytical chemistry · 2011Article
- Expecting the unexpected: nucleic acid-based diagnosis and discovery of emerging viruses.Expert review of molecular diagnostics · 2011Article
- A microbial detection array (MDA) for viral and bacterial detection.BMC genomics · 2010Article
- VIPR: A probabilistic algorithm for analysis of microbial detection microarrays.BMC bioinformatics · 2010Article
- Nonparametric methods for the analysis of single-color pathogen microarrays.BMC bioinformatics · 2010Article
- Viral nucleic acids in live-attenuated vaccines: detection of minority variants and an adventitious virus.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA microarrays used as 'genomic sensors' have great potential in clinical diagnostics. Biases inherent in random PCR-amplification, cross-hybridization effects, and inadequate microarray analysis, however, limit detection sensitivity and specificity. Here, we have studied the relationships between viral amplification efficiency, hybridization signal, and target-probe annealing specificity using a customized microarray platform. Novel features of this platform include the development of a robust algorithm that accurately predicts PCR bias during DNA amplification and can be used to improve PCR primer design, as well as a powerful statistical concept for inferring pathogen identity from probe recognition signatures. Compared to real-time PCR, the microarray platform identified pathogens with 94% accuracy (76% sensitivity and 100% specificity) in a panel of 36 patient specimens. Our findings show that microarrays can be used for the robust and accurate diagnosis of pathogens, and further substantiate the use of microarray technology in clinical diagnostics.
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Registered trials
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