ArticleParasitology research2007
Detection of Toxoplasma gondii in water by an immunomagnetic separation method targeting the sporocysts.
Article in Parasitology research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks withMicroorganisms · 2022Review
- Immunomagnetic separation ofFrontiers in veterinary science · 2022Article
- rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts.Scientific reports · 2020Article
- Cell type- and species-specific host responses to Toxoplasma gondii and its near relatives.International journal for parasitology · 2020Review
- Environmental transmission ofFood and waterborne parasitology · 2019Review
- Importance of serological cross-reactivity among Toxoplasma gondii, Hammondia spp., Neospora spp., Sarcocystis spp. and Besnoitia besnoiti.Parasitology · 2017Review
- Mechanics of the Toxoplasma gondii oocyst wall.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Molecules to modeling: Toxoplasma gondii oocysts at the human-animal-environment interface.Comparative immunology, microbiology and infectious diseases · 2013Review
- The impact of the waterborne transmission of Toxoplasma gondii and analysis efforts for water detection: an overview and update.Environmental science and pollution research international · 2013Review
- The first detection of Toxoplasma gondii DNA in environmental fruits and vegetables samples.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Epidemiology of and diagnostic strategies for toxoplasmosis.Clinical microbiology reviews · 2012Review
- Determining UV inactivation of Toxoplasma gondii oocysts by using cell culture and a mouse bioassay.Applied and environmental microbiology · 2010Article
- Detection of Toxoplasma gondii oocysts in environmental soil samples using molecular methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Detection of Toxoplasma gondii oocysts in water sample concentrates by real-time PCR.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An immunomagnetic separation (IMS) method was developed to detect Toxoplasma gondii in fresh waters by using the monoclonal antibody 4B6 targeting the sporocyst wall of T. gondii, Hammondia hammondi, Hammondia heydorni, and Neospora caninum. Water concentrates obtained by filtering 10- to 20-l samples samples were spiked with Toxoplasma oocysts, sonicated to release the sporocysts, and analyzed by IMS-4B6. Mean sporocyst recoveries were 74.5 +/- 5.3% in drinking water, 30.6 +/- 2.4 and 37.1 +/- 3.2% in surface waters, and 81.6 +/- 2.1% in IMS buffer. Then, this IMS method was integrated in a multistep procedure (i.e., filtration, IMS, immunofluorescence and autofluorescence) to detect Toxoplasma in unspiked and spiked water samples (10-30 l) of various qualities. Sporocyst recoveries ranged from 14.4 to 44.7% in drinking water samples spiked with 1-10 oocysts/l, and from 17.8 to 32.5% in surface water samples spiked with 10 oocysts/l. Sporocysts were not detected in 25 unspiked water samples. A sporocyst-like structure was seen in one of these unspiked samples, but its coccidian nature could not be proved by three polymerase chain reaction (PCR) methods targeting sequences of coccidian small and large subunit rRNA genes and Toxoplasma repetitive elements. In conclusion, IMS-4B6 is relevant for the detection of Toxoplasma in water generating small concentrates (<1 ml). Due to 4B6 cross-reactions, a PCR would be useful to further characterize coccidian sporocysts found microscopically.
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