ArticleBlood2007
Cytoplasmic remodeling of erythrocyte raft lipids during infection by the human malaria parasite Plasmodium falciparum.
Article in Blood, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
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- Protein Profiling of Malaria-Derived Extracellular Vesicles Reveals Distinct Subtypes.Membranes · 2022Article
- The role of cholesterol in invasion and growth of malaria parasites.Frontiers in cellular and infection microbiology · 2022Review
- Glycosphingolipid GM3 is localized in both exoplasmic and cytoplasmic leaflets of Plasmodium falciparum malaria parasite plasma membrane.Scientific reports · 2021Article
- 4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation.Nature communications · 2021Article
- Real-time cholesterol sorting in Plasmodium falciparum-erythrocytes as revealed by 3D label-free imaging.Scientific reports · 2020Article
- Elevated Cholesterol in themBio · 2017Article
- Erythropoietin Receptor Signaling and Lipid Rafts.Vitamins and hormones · 2017Review
- Host cell remodeling by pathogens: the exomembrane system in Plasmodium-infected erythrocytes.FEMS microbiology reviews · 2016Review
- Physicochemical Aspects of the Plasmodium chabaudi-Infected Erythrocyte.BioMed research international · 2015Article
- In vivo splenic clearance correlates with in vitro deformability of red blood cells from Plasmodium yoelii-infected mice.Infection and immunity · 2014Article
- Inward cholesterol gradient of the membrane system in P. falciparum-infected erythrocytes involves a dilution effect from parasite-produced lipids.Biology open · 2014Article
- Disruption of lipid rafts interferes with the interaction of Toxoplasma gondii with macrophages and epithelial cells.BioMed research international · 2014Article
- Proteomic analysis of detergent-resistant membrane microdomains in trophozoite blood stage of the human malaria parasite Plasmodium falciparum.Molecular & cellular proteomics : MCP · 2013Article
- Sink or swim: lipid rafts in parasite pathogenesis.Trends in parasitology · 2012Review
- Modifications in erythrocyte membrane zeta potential by Plasmodium falciparum infection.Experimental parasitology · 2012Article
- Extracellular ATP triggers proteolysis and cytosolic Ca²⁺ rise in Plasmodium berghei and Plasmodium yoelii malaria parasites.Malaria journal · 2012Article
- Plasmodium falciparum infection-induced changes in erythrocyte membrane proteins.Parasitology research · 2012Article
- Intruders below the radar: molecular pathogenesis of Bartonella spp.Clinical microbiology reviews · 2012Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Studies of detergent-resistant membrane (DRM) rafts in mature erythrocytes have facilitated identification of proteins that regulate formation of endovacuolar structures such as the parasitophorous vacuolar membrane (PVM) induced by the malaria parasite Plasmodium falciparum. However, analyses of raft lipids have remained elusive because detergents interfere with lipid detection. Here, we use primaquine to perturb the erythrocyte membrane and induce detergent-free buoyant vesicles, which are enriched in cholesterol and major raft proteins flotillin and stomatin and contain low levels of cytoskeleton, all characteristics of raft microdomains. Lipid mass spectrometry revealed that phosphatidylethanolamine and phosphatidylglycerol are depleted in endovesicles while phosphoinositides are highly enriched, suggesting raft-based endovesiculation can be achieved by simple (non-receptor-mediated) mechanical perturbation of the erythrocyte plasma membrane and results in sorting of inner leaflet phospholipids. Live-cell imaging of lipid-specific protein probes showed that phosphatidylinositol (4,5) bisphosphate (PIP(2)) is highly concentrated in primaquine-induced vesicles, confirming that it is an erythrocyte raft lipid. However, the malarial PVM lacks PIP(2), although another raft lipid, phosphatidylserine, is readily detected. Thus, different remodeling/sorting of cytoplasmic raft phospholipids may occur in distinct endovacuoles. Importantly, erythrocyte raft lipids recruited to the invasion junction by mechanical stimulation may be remodeled by the malaria parasite to establish blood-stage infection.
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Registered trials
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