Evidence map›Paper›PMID 17526790›Full record

ArticleApplied and environmental microbiology2007

Nucleotide sequence of plasmid pCNB1 from comamonas strain CNB-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation.

Ying-Fei Ma, Jian-Feng Wu, Sheng-Yue Wang, Cheng-Ying Jiang, Yun Zhang, Su-Wei Qi, Lei Liu, Guo-Ping Zhao, Shuang-Jiang Liu

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.8field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 57 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Ying-Fei MaState Key Laboratory of Microbial Resource at Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100080, People's Republic of China.
Jian-Feng Wu
Sheng-Yue Wang
Cheng-Ying Jiang
Yun Zhang
Su-Wei Qi
Lei Liu
Guo-Ping Zhao
Shuang-Jiang Liu
Czech Academy of Sciences, Institute of Microbiology · CZChinese National Human Genome Center at Shanghai · CNShanghai Institutes for Biological Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The nucleotide sequence of a new plasmid pCNB1 from Comamonas sp. strain CNB-1 that degrades 4-chloronitrobenzene (4CNB) was determined. pCNB1 belongs to the IncP-1beta group and is 91,181 bp in length. A total of 95 open reading frames appear to be involved in (i) the replication, maintenance, and transfer of pCNB1; (ii) resistance to arsenate and chromate; and (iii) the degradation of 4CNB. The 4CNB degradative genes and arsenate resistance genes were located on an extraordinarily large transposon (44.5 kb), proposed as TnCNB1. TnCNB1 was flanked by two IS1071 elements and represents a new member of the composite I transposon family. The 4CNB degradative genes within TnCNB1 were separated by various truncated genes and genetic homologs from other DNA molecules. Genes for chromate resistance were located on another transposon that was similar to the Tn21 transposon of the class II replicative family that is frequently responsible for the mobilization of mercury resistance genes. Resistance to arsenate and chromate were experimentally confirmed, and transcriptions of arsenate and chromate resistance genes were demonstrated by reverse transcription-PCR. These results described a new member of the IncP-1beta plasmid family, and the findings suggest that gene deletion and acquisition as well as genetic rearrangement of DNA molecules happened during the evolution of the 4CNB degradation pathway on pCNB1.

Indexed as

Anti-Bacterial AgentsArsenatesBase SequenceBiodegradation, EnvironmentalChromatesComamonasDNA, BacterialDNA ReplicationDNA Transposable ElementsDrug Resistance, BacterialEvolution, MolecularGene ExpressionGene OrderMetabolic Networks and PathwaysMolecular Sequence DataNitrobenzenes4-chloronitrobenzeneAnti-Bacterial AgentsArsenatesarsenic acidChromatesDNA, BacterialDNA Transposable ElementsNitrobenzenesRNA, BacterialRNA, Messenger

Identifiers

PMID17526790
PMCPMC1932830
OpenAlexW2125212058

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.