ArticleMolecular and cellular biology2007
Antisense intergenic transcription precedes Igh D-to-J recombination and is controlled by the intronic enhancer Emu.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 82 citations in OpenAlex.
- Interplay between CTCF-binding and CTCF-lacking regulatory elements in generating an architectural stripe at the Igh locus.Nature communications · 2025Article
- Rare Transcripts Quantification by Strand-Specific RT-qPCR.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Exuberant long noncoding RNA expression may sculpt Igh locus topology.Frontiers in immunology · 2025Article
- Lineage- and stage-specific activity of antigen receptor gene enhancers during lymphocyte development.Frontiers in epigenetics and epigenomics · 2024Review
- Intra- and interchromosomal contact mapping reveals the Igh locus has extensive conformational heterogeneity and interacts with B-lineage genes.Cell reports · 2023Article
- An Igh distal enhancer modulates antigen receptor diversity by determining locus conformation.Nature communications · 2023Article
- The role of chromatin loop extrusion in antibody diversification.Nature reviews. Immunology · 2022Review
- RNA exosome drives early B cell development via noncoding RNA processing mechanisms.Science immunology · 2022Article
- Promoter Proximity Defines Mutation Window for VJournal of immunology (Baltimore, Md. : 1950) · 2022Article
- IL-7R signaling activates widespread VCell reports · 2021Article
- Review
- Recombination may occur in the absence of transcription in the immunoglobulin heavy chain recombination centre.Nucleic acids research · 2020Article
- Dynamic 3D Locus Organization and Its Drivers Underpin Immunoglobulin Recombination.Frontiers in immunology · 2020Review
- Article
- New insights emerge as antibody repertoire diversification meets chromosome conformation.F1000Research · 2019Review
- The RAG-2 Inhibitory Domain Gates Accessibility of the V(D)J Recombinase to Chromatin.Molecular and cellular biology · 2018Article
- Non-coding Class Switch Recombination-Related Transcription in Human Normal and Pathological Immune Responses.Frontiers in immunology · 2018Article
- RNA Exosome and Non-coding RNA-Coupled Mechanisms in AID-Mediated Genomic Alterations.Journal of molecular biology · 2017Review
- Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination.Cell reports · 2016Article
- Sequential activation and distinct functions for distal and proximal modules within the IgH 3' regulatory region.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
V(D)J recombination is believed to be regulated by alterations in chromatin accessibility to the recombinase machinery, but the mechanisms responsible remain unclear. We previously proposed that antisense intergenic transcription, activated throughout the mouse Igh VH region in pro-B cells, remodels chromatin for VH-to-DJH recombination. Using RNA fluorescence in situ hybridization, we now show that antisense intergenic transcription occurs throughout the Igh DHJH region before D-to-J recombination, indicating that this is a widespread process in V(D)J recombination. Transcription initiates near the Igh intronic enhancer Emu and is abrogated in mice lacking this enhancer, indicating that Emu regulates DH antisense transcription. Emu was recently demonstrated to regulate DH-to-JH recombination of the Igh locus. Together, these data suggest that Emu controls DH-to-JH recombination by activating this form of germ line Igh transcription, thus providing a long-range, processive mechanism by which Emu can regulate chromatin accessibility throughout the DH region. In contrast, Emu deletion has no effect on VH antisense intergenic transcription, which is rarely associated with DH antisense transcription, suggesting differential regulation and separate roles for these processes at sequential stages of V(D)J recombination. These results support a directive role for antisense intergenic transcription in enabling access to the recombination machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.