ArticleJournal of bacteriology2007
Drug-induced conformational changes in multidrug efflux transporter AcrB from Haemophilus influenzae.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Environment-dependent epistasis shapes adaptation to fluctuating resources.bioRxiv : the preprint server for biology · 2026Article
- Membranome-based identification of amino acid substitution in Haemophilus influenzae multidrug efflux pump HmrM for reduced chloramphenicol susceptibility.Archives of microbiology · 2024Article
- Copper Efflux System Required in Murine Lung Infection by Haemophilus influenzae Composed of a Canonical ATPase Gene and Tandem Chaperone Gene Copies.Infection and immunity · 2023Article
- Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli.Scientific reports · 2020Article
- Phylogenetic and functional characterisation of theCommunications biology · 2019Article
- Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model.Journal of microbiology (Seoul, Korea) · 2015Review
- The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.Clinical microbiology reviews · 2015Review
- Article
- Structure and mechanism of RND-type multidrug efflux pumps.Advances in enzymology and related areas of molecular biology · 2011Review
- Substrate path in the AcrB multidrug efflux pump of Escherichia coli.Molecular microbiology · 2010Article
- Review
- Mechanisms of RND multidrug efflux pumps.Biochimica et biophysica acta · 2009Review
- Assembly and transport mechanism of tripartite drug efflux systems.Biochimica et biophysica acta · 2009Review
- Efflux pump, the masked side of beta-lactam resistance in Klebsiella pneumoniae clinical isolates.PloS one · 2009Article
- Efflux pump gene hefA of Helicobacter pylori plays an important role in multidrug resistance.World journal of gastroenterology · 2008Article
- Fitting periplasmic membrane fusion proteins to inner membrane transporters: mutations that enable Escherichia coli AcrA to function with Pseudomonas aeruginosa MexB.Journal of bacteriology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In gram-negative bacteria, transporters belonging to the resistance-nodulation-cell division (RND) superfamily of proteins are responsible for intrinsic multidrug resistance. Haemophilus influenzae, a gram-negative pathogen causing respiratory diseases in humans and animals, constitutively produces the multidrug efflux transporter AcrB (AcrB(HI)). Similar to other RND transporters AcrB(HI) associates with AcrA(HI), the periplasmic membrane fusion protein, and the outer membrane channel TolC(HI). Here, we report that AcrAB(HI) confers multidrug resistance when expressed in Escherichia coli and requires for its activity the E. coli TolC (TolC(EC)) protein. To investigate the intracellular dynamics of AcrAB(HI), single cysteine mutations were constructed in AcrB(HI) in positions previously identified as important for substrate recognition. The accessibility of these strategically positioned cysteines to the hydrophilic thiol-reactive fluorophore fluorescein-5-maleimide (FM) was studied in vivo in the presence of various substrates of AcrAB(HI) and in the presence or absence of AcrA(HI) and TolC(EC). We report that the reactivity of specific cysteines with FM is affected by the presence of some but not all substrates. Our results suggest that substrates induce conformational changes in AcrB(HI).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.